DNase-activatable fluorescence probes visualizing the degradation of exogenous DNA in living cells
文献类型：期刊论文
| 作者 | Gong, Ping; Shi, Bihua; Zhang, Pengfei; Hu, Dehong; Zheng, Mingbin; Zheng, Cuifang; Gao, Duyang; Cai, Lintao |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2012 |
| 英文摘要 | This work presents a method to visualize the degradation of exogenous DNA in living cells using a novel type of activatable fluorescence imaging probe. Deoxyribonuclease (DNase)-activatable fluorescence probes (DFProbes) are composed of double strands deoxyribonucleic acid (dsDNA) which is labeled with fluorophore (ROX or Cy3) and quencher on the end of one of its strands, and stained with SYBR Green I. In the absence of DNase, DFProbes produce thegreen fluorescence signal of SYBR Green I. In the presence of DNase, SYBR Green I is removed from the DFProbes and the labeled fluorophore is separated from the quencher owing to the degradation of DFProbes by DNase, resulting in the decrease of the green fluorescence signal and the occurrence of a redfluorescence signal due to fluorescence resonance energy transfer (FRET). DNase in biological samples was detected using DFProbes and the fluorescenceimaging in living cells was performed using DFprobe-modified Au nanoparticles. The results show that DFProbes have good responses to DNase, and can clearly visualize the degradation of exogenous DNA in cells in real time. The well-designed probes might be useful in tracing the dynamic changes ofexogenous DNA and nanocarriers in vitro and in vivo. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.rsc.org/en/Content/ArticleLanding/2012/NR/c2nr12005d#!divAbstract |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4283]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | NANOSCALE |
| 推荐引用方式 GB/T 7714 | Gong, Ping,Shi, Bihua,Zhang, Pengfei,et al. DNase-activatable fluorescence probes visualizing the degradation of exogenous DNA in living cells[J]. NANOSCALE,2012. |
| APA | Gong, Ping.,Shi, Bihua.,Zhang, Pengfei.,Hu, Dehong.,Zheng, Mingbin.,...&Cai, Lintao.(2012).DNase-activatable fluorescence probes visualizing the degradation of exogenous DNA in living cells.NANOSCALE. |
| MLA | Gong, Ping,et al."DNase-activatable fluorescence probes visualizing the degradation of exogenous DNA in living cells".NANOSCALE (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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