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Homogeneous bioluminescence detection of biomolecules using target-triggered hybridization chain reaction-mediated ligation without luciferase label
文献类型：期刊论文
| 作者 | Xu, Qinfeng; Zhu, Guichi; Zhang, Chun-yang |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013 |
| 英文摘要 | We develop a new homogeneous method for sensitive detection of various biomolecules on the basis of bioluminescence monitoring the released AMP from the target-triggered hybridization chain reaction-mediated ligation. The introduction of hybridization chain reaction not only improves the sensitivity of DNA assay, but also facilitates the sensitive detection of proteins by designing specific aptamer triggers, providing a universally amplified platform for simultaneousdetection of different kinds of biomolecules. Importantly, this bioluminescence assay employs the target-dependent ATP from the ligation byproduct of AMP as the reporter without the requirement for the sophisticated luciferase manipulation, complicated immobilization, and separation steps. The proposed method has significant advantages of simplicity, high sensitivity, low cost, and high throughput, and holds a great promise for practical point-of-care applications. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.acs.org/doi/abs/10.1021/ac401334r |
| 语种 | 英语 |
| WOS记录号 | WOS:000322059600053 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5155]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | ANALYTICAL CHEMISTRY |
| 推荐引用方式 GB/T 7714 | Xu, Qinfeng,Zhu, Guichi,Zhang, Chun-yang. Homogeneous bioluminescence detection of biomolecules using target-triggered hybridization chain reaction-mediated ligation without luciferase label[J]. ANALYTICAL CHEMISTRY,2013. |
| APA | Xu, Qinfeng,Zhu, Guichi,&Zhang, Chun-yang.(2013).Homogeneous bioluminescence detection of biomolecules using target-triggered hybridization chain reaction-mediated ligation without luciferase label.ANALYTICAL CHEMISTRY. |
| MLA | Xu, Qinfeng,et al."Homogeneous bioluminescence detection of biomolecules using target-triggered hybridization chain reaction-mediated ligation without luciferase label".ANALYTICAL CHEMISTRY (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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