Hepatic response to chronic hypoxia in experimental rat model through HIF-1 alpha, activator protein-1 and NF-kappa B
文献类型：期刊论文
| 作者 | Thomas Y.H. Lau; Jia Xiao; Emily C. Liong; Linchuan Liao; Tung Ming Leung; Amin A. Nanji; Man Lung Fung; George L. Tipoe |
| 刊名 | HISTOLOGY AND HISTOPATHOLOGY
 |
| 出版日期 | 2013 |
| 英文摘要 | Chronic liver diseases are commonly associated with tissue hypoxia that may cause inflammation, oxidative stress, liver cell injury and increased nuclear transcriptional regulation. The hepatic response to chronic hypoxia at the molecular level has not yet been clearly understood until now. The aim of this study is to investigate whether nuclear transcription factors [hypoxia-inducible factor-1 (HIF-1α), activator protein-1 (AP-1), nuclear factor-kappa B (NF-κB)] exhibit activity changes during hepatic response to chronic hypoxia. Blood and liver samples were collected from adult Sprague-Dawley rats living in atmospheric air or 10% oxygen for four weeks. Levels of serum alanine aminotransferase (ALT), 8-isoprostane and nitrotyrosine were measured. The activities of nuclear transcription factors and the expression of downstream genes (iNOS, eNOS, ET-1 and VEGF) were measured using RT-PCR, Western blotting and Gel shift analysis. Results showed that serum ALT level, 8-isoprostane level and formation of nitrotyrosine were within normal range at all time-points. In the hypoxic liver, DNA-binding activities of HIF-1α, NF-κB and AP-1 increased significantly. Expression levels of iNOS, VEGF and ET-1 progressively increased from day 7 to day 28. eNOS was also elevated in the hypoxic liver. In conclusion, our study suggests that increased activity of HIF-1α, AP-1 and NF-κB may partly play a significant role in the hepatic response to oxidative stress and liver injury under chronic hypoxia. The increased expression of VEGF, ET-1, iNOS and eNOS may be partly due to the compensatory mechanism in the vascular beds of the liver in response to chronic hypoxia. |
| 收录类别 | SCI |
| 原文出处 | http://www.hh.um.es/Abstracts/Vol_28/28_4/28_4_463.htm |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5182]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | HISTOLOGY AND HISTOPATHOLOGY |
| 推荐引用方式 GB/T 7714 | Thomas Y.H. Lau,Jia Xiao,Emily C. Liong,et al. Hepatic response to chronic hypoxia in experimental rat model through HIF-1 alpha, activator protein-1 and NF-kappa B[J]. HISTOLOGY AND HISTOPATHOLOGY,2013. |
| APA | Thomas Y.H. Lau.,Jia Xiao.,Emily C. Liong.,Linchuan Liao.,Tung Ming Leung.,...&George L. Tipoe.(2013).Hepatic response to chronic hypoxia in experimental rat model through HIF-1 alpha, activator protein-1 and NF-kappa B.HISTOLOGY AND HISTOPATHOLOGY. |
| MLA | Thomas Y.H. Lau,et al."Hepatic response to chronic hypoxia in experimental rat model through HIF-1 alpha, activator protein-1 and NF-kappa B".HISTOLOGY AND HISTOPATHOLOGY (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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