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Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8(+) T-cell Responses
文献类型：期刊论文
| 作者 | Wynette M Dietz; Nicole E B Skinner; Sara E Hamilton; Michelle D Jund; Suzanne M Heitfeld; Adam J Litterman; Patrick Hwu; Zhi-Ying Chen; Andres M Salazar; John R Ohlfest |
| 刊名 | MOLECULAR THERAPY
 |
| 出版日期 | 2013 |
| 英文摘要 | Clinical trials reveal that plasmid DNA (pDNA)–based gene delivery must be improved to realize its potential to treat human disease. Current pDNA platforms suffer from brief transgene expression, primarily due to the spread of transcriptionally repressive chromatin initially deposited on plasmid bacterial backbone sequences. Minicircle (MC) DNA lacks plasmid backbone sequences and correspondingly confers higher levels of sustained transgene expression upon delivery, accounting for its success in preclinical gene therapy models. In this study, we show for the first time that MC DNA also functions as a vaccine platform. We used a luciferase reporter transgene to demonstrate that intradermal delivery of MC DNA, relative to pDNA, resulted in significantly higher and persistent levels of luciferase expression in mouse skin. Next, we immunized mice intradermally with DNA encoding a peptide that, when presented by the appropriate major histocompatibility complex class I molecule, was recognized by endogenous CD8+ T cells. Finally, immunization with peptide-encoding MC DNA, but not the corresponding full-length (FL) pDNA, conferred significant protection in mice challenged with Listeria monocytogenes expressing the model peptide. Together, our results suggest intradermal delivery of MC DNA may prove more efficacious for prophylaxis than traditional pDNA vaccines. |
| 收录类别 | SCI |
| 原文出处 | http://www.nature.com/mt/journal/v21/n8/full/mt201385a.html |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5183]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | MOLECULAR THERAPY |
| 推荐引用方式 GB/T 7714 | Wynette M Dietz,Nicole E B Skinner,Sara E Hamilton,et al. Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8(+) T-cell Responses[J]. MOLECULAR THERAPY,2013. |
| APA | Wynette M Dietz.,Nicole E B Skinner.,Sara E Hamilton.,Michelle D Jund.,Suzanne M Heitfeld.,...&Mark J Osborn.(2013).Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8(+) T-cell Responses.MOLECULAR THERAPY. |
| MLA | Wynette M Dietz,et al."Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8(+) T-cell Responses".MOLECULAR THERAPY (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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