Bioreducible alginate-poly (ethylenimine) nanogels as an antigen-delivery system robustly enhance vaccine-elicited humoral and cellular immune responses
文献类型：期刊论文
| 作者 | Li, Ping; Luo, Zichao; Liu, Peng; Gao, Ningning; Zhang, Yijuan; Pan, Hong; Liu, Lanlan; Wang, Ce; Cai, Lintao; Ma, Yifan |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
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| 出版日期 | 2013 |
| 英文摘要 | Although polysaccharide nanogels have emerged as a novel antigen delivery system for vaccine development, whether modulating the redox sensitivity ofnanogels could improve vaccine efficacy remains unclear. In the present study, we generated bioreducible cationic alginate-polyethylenimine (PEI) nanogels asa novel vaccine delivery system. Briefly, nanogels were prepared by the electrostatic interaction of negatively charged alginate sodium with branched PEI2k, followed by disulfide cross-linking to generate bioreducible nanogels (AP-SS). The AP-SS nanogels demonstrated great antigen-loading capacity and minimal cytotoxicity. The in vitro study showed that reducible AP-SS nanogels not only facilitated antigen uptake by mouse bone marrow dendritic cells (BMDCs), but also promoted intracellular antigen degradation and cytosolic release. Moreover, AP-SS nanogels significantly enhanced both MHC class I and II antigenpresentation by BMDCs. Compared with the non-reducible nanogels, AP-SS nanogels more potently enhanced vaccine-induced antibody production and CD8(+) T cell-mediated tumor cell lysis. Hence, the bioreducible alginate-PEI nanogels could serve as a potent adjuvant to improve vaccine-elicited humoraland cellular immune responses. (C) 2013 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0168365913001685 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5187]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | JOURNAL OF CONTROLLED RELEASE |
| 推荐引用方式 GB/T 7714 | Li, Ping,Luo, Zichao,Liu, Peng,et al. Bioreducible alginate-poly (ethylenimine) nanogels as an antigen-delivery system robustly enhance vaccine-elicited humoral and cellular immune responses[J]. JOURNAL OF CONTROLLED RELEASE,2013. |
| APA | Li, Ping.,Luo, Zichao.,Liu, Peng.,Gao, Ningning.,Zhang, Yijuan.,...&Ma, Yifan.(2013).Bioreducible alginate-poly (ethylenimine) nanogels as an antigen-delivery system robustly enhance vaccine-elicited humoral and cellular immune responses.JOURNAL OF CONTROLLED RELEASE. |
| MLA | Li, Ping,et al."Bioreducible alginate-poly (ethylenimine) nanogels as an antigen-delivery system robustly enhance vaccine-elicited humoral and cellular immune responses".JOURNAL OF CONTROLLED RELEASE (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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