Amorphous Calcium Carbonate Precipitation by Cellular Biomineralization in Mantle Cell Cultures of Pinctada fucata
文献类型：期刊论文
| 作者 | Xiang Liang; Kong Wei; Su Jingtan; Liang Jian; Zhang Guiyou; Xie Liping; Zhang Rongqing |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 英文摘要 | The growth of molluscan shell crystals is generally thought to be initiated from the extrapallial fluid by matrix proteins, however, the cellular mechanisms of shell formation pathway remain unknown. Here, we first report amorphous calcium carbonate (ACC) precipitation by cellular biomineralization in primary mantle cell cultures of Pinctada fucata. Through real-time PCR and western blot analyses, we demonstrate that mantle cells retain the ability to synthesize and secrete ACCBP, Pif80 and nacrein in vitro. In addition, the cells also maintained high levels of alkaline phosphatase and carbonic anhydrase activity, enzymes responsible for shell formation. On the basis of polarized light microscopy and scanning electron microscopy, we observed intracellular crystals production by mantle cells in vitro. Fourier transform infrared spectroscopy and X-ray diffraction analyses revealed the crystals to be ACC, and de novo biomineralization was confirmed by following the incorporation of Sr into calcium carbonate. Our results demonstrate the ability of mantle cells to perform fundamental biomineralization processes via amorphous calcium carbonate, and these cells may be directly involved in pearl oyster shell formation. |
| 收录类别 | SCI |
| 原文出处 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113150 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6081]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | PLOS ONE |
| 推荐引用方式 GB/T 7714 | Xiang Liang,Kong Wei,Su Jingtan,et al. Amorphous Calcium Carbonate Precipitation by Cellular Biomineralization in Mantle Cell Cultures of Pinctada fucata[J]. PLOS ONE,2014. |
| APA | Xiang Liang.,Kong Wei.,Su Jingtan.,Liang Jian.,Zhang Guiyou.,...&Zhang Rongqing.(2014).Amorphous Calcium Carbonate Precipitation by Cellular Biomineralization in Mantle Cell Cultures of Pinctada fucata.PLOS ONE. |
| MLA | Xiang Liang,et al."Amorphous Calcium Carbonate Precipitation by Cellular Biomineralization in Mantle Cell Cultures of Pinctada fucata".PLOS ONE (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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