Noninvasive Visualization of Respiratory Viral Infection Using Bioorthogonal conjugated Near-Infrared Emitting Quantum Dots
文献类型：期刊论文
| 作者 | Pan, Hong; Zhang, Pengfei; Gao, Duyang; Zhang, Yijuan; Li, Ping; Liu, Lanlan; Wang, Ce; Wang, Hanzhong; Ma, Yifan; Cai, Lintao |
| 刊名 | ACS NANO
 |
| 出版日期 | 2014 |
| 英文摘要 | Highly pathogenic avian influenza A viruses are emerging pandemic threats in human beings. Monitoring the in vivo dynamics of avian influenza viruses is extremely important for understanding viral pathogenesis and developing antiviral drugs. Although a number of technologies have been applied for tracking viralinfection in vivo, most of them are laborious with unsatisfactory detection sensitivity. Herein we labeled avian influenza H5N1 pseudotype virus (H5N1p) withnear-infrared (NIR)-emitting QDs by bioorthogonal chemistry. The conjugation of QDs onto H5N1p was highly efficient with superior stability both in vitro and in vivo. Furthermore, QD-labeled H5N1p (QD-H5N1p) demonstrated bright and sustained fluorescent signals in mouse lung tissues, allowing us to visualizerespiratory viral infection in a noninvasive and real-time manner. The fluorescence signals of QD-H5N1p in lung were correlated with the severity of virusinfection and significantly attenuated by antiviral agents, such as oseltamivir carboxylate and mouse antiserum against H5N1p. The biodistribution of QD-H5N1p in lungs and other organs could be easily quantified by measuring fluorescent signals and cadmium concentration of virus-conjugated QDs in tissues. Hence, virus labeling with NIR QDs provides a simple, reliable, and quantitative strategy for tracking respiratory viral infection and for antiviral drug screening. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.acs.org/doi/abs/10.1021/nn501028b |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6082]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | ACS NANO |
| 推荐引用方式 GB/T 7714 | Pan, Hong,Zhang, Pengfei,Gao, Duyang,et al. Noninvasive Visualization of Respiratory Viral Infection Using Bioorthogonal conjugated Near-Infrared Emitting Quantum Dots[J]. ACS NANO,2014. |
| APA | Pan, Hong.,Zhang, Pengfei.,Gao, Duyang.,Zhang, Yijuan.,Li, Ping.,...&Cai, Lintao.(2014).Noninvasive Visualization of Respiratory Viral Infection Using Bioorthogonal conjugated Near-Infrared Emitting Quantum Dots.ACS NANO. |
| MLA | Pan, Hong,et al."Noninvasive Visualization of Respiratory Viral Infection Using Bioorthogonal conjugated Near-Infrared Emitting Quantum Dots".ACS NANO (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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