Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies
文献类型：期刊论文
| 作者 | Zhao Botao; Yang Dehua; Jiang Jing; Li Jinsong; Fan Chunsun; Huang Menggui; Fan Yi; Jin Yan; Jin Youxin |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2014 |
| 英文摘要 | Background: Reprogrammed cells, including induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cells (NT-ESCs), are similar in many respects to natural embryonic stem cells (ESCs). However, previous studies have demonstrated that iPSCs retain a gene expression signature that is unique from that of ESCs, including differences in microRNA (miRNA) expression, while NT-ESCs are more faithfully reprogrammed cells and have better developmental potential compared with iPSCs. Results: We focused on miRNA expression and explored the difference between ESCs and reprogrammed cells, especially ESCs and NT-ESCs. We also compared the distinct expression patterns among iPSCs, NT-ESCs and NT-iPSCs. The results demonstrated that reprogrammed cells (iPSCs and NT-ESCs) have unique miRNA expression patterns compared with ESCs. The comparison of differently reprogrammed cells (NT-ESCs, NT-iPSCs and iPSCs) suggests that several miRNAs have key roles in the distinct developmental potential of reprogrammed cells. Conclusions: Our data suggest that miRNAs play a part in the difference between ESCs and reprogrammed cells, as well as between MEFs and pluripotentcells. The variation of miRNA expression in reprogrammed cells derived using different reprogramming strategies suggests different characteristics induced bynuclear transfer and iPSC generation, as well as different developmental potential among NT-ESCs, iPSCs and NT-iPSCs. |
| 收录类别 | SCI |
| 原文出处 | http://www.biomedcentral.com/1471-2164/15/488 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6091]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | BMC GENOMICS |
| 推荐引用方式 GB/T 7714 | Zhao Botao,Yang Dehua,Jiang Jing,et al. Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies[J]. BMC GENOMICS,2014. |
| APA | Zhao Botao.,Yang Dehua.,Jiang Jing.,Li Jinsong.,Fan Chunsun.,...&Jin Youxin.(2014).Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies.BMC GENOMICS. |
| MLA | Zhao Botao,et al."Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies".BMC GENOMICS (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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