Pyruvate-associated acid resistance in bacteria.
文献类型：期刊论文
| 作者 | Wu, Jianting; Li, Yannan; Cai, Zhiming; Jin, Ye |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2014 |
| 英文摘要 | Glucose confers acid resistance on exponentially growing bacteria by repressing formation of the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex and consequently activating acid resistance genes. Therefore, in a glucose-rich growth environment, bacteria are capable of resisting acidic stresses due to low levels of cAMP-CRP. Here we reveal a second mechanism for glucose-conferred acid resistance. We show that glucose induces acid resistance inexponentially growing bacteria through pyruvate, the glycolysis product. Pyruvate and/or the downstream metabolites induce expression of the small noncoding RNA (sncRNA) Spot42, and the sncRNA, in turn, activates expression of the master regulator of acid resistance, RpoS. In contrast to glucose, pyruvate has little effect on levels of the cAMP-CRP complex and does not require the complex for its effects on acid resistance. Another important difference between glucose and pyruvate is that pyruvate can be produced by bacteria. This means that bacteria have the potential to protect themselves from acidic stresses by controlling glucose-derived generation of pyruvate, pyruvate-acetate efflux, or reversion from acetate to pyruvate. We tested this possibility by shutting downpyruvate-acetate efflux and found that the resulting accumulation of pyruvate elevated acid resistance. Many sugars can be broken into glucose, and the subsequent glycolysis generates pyruvate. Therefore, pyruvate-associated acid resistance is not confined to glucose-grown bacteria but is functional inbacteria grown on various sugars. |
| 收录类别 | SCI |
| 原文出处 | http://aem.asm.org/content/80/14/4108 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6114]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
| 推荐引用方式 GB/T 7714 | Wu, Jianting,Li, Yannan,Cai, Zhiming,et al. Pyruvate-associated acid resistance in bacteria.[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014. |
| APA | Wu, Jianting,Li, Yannan,Cai, Zhiming,&Jin, Ye.(2014).Pyruvate-associated acid resistance in bacteria..APPLIED AND ENVIRONMENTAL MICROBIOLOGY. |
| MLA | Wu, Jianting,et al."Pyruvate-associated acid resistance in bacteria.".APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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