Identification of Novel Knockout Targets for Improving Terpenoids Biosynthesis inSaccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Sun, Zhiqiang; Meng, Hailin; Li, Jing; Wang, Jianfeng; Li, Qian; Wang, Yong; Zhang, Yansheng |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 英文摘要 | Many terpenoids have important pharmacological activity and commercial value; however, application of these terpenoids is often limited by problems associated with the production of sufficient amounts of these molecules. The use of Saccharomyces cerevisiae (S. cerevisiae) for the production ofheterologous terpenoids has achieved some success. The objective of this study was to identify S. cerevisiae knockout targets for improving the synthesis ofheterologous terpeniods. On the basis of computational analysis of the S. cerevisiae metabolic network, we identified the knockout sites with the potential to promote terpenoid production and the corresponding single mutant was constructed by molecular manipulations. The growth rates of these strains were measured and the results indicated that the gene deletion had no adverse effects. Using the expression of amorphadiene biosynthesis as a testing model, the gene deletion was assessed for its effect on the production of exogenous terpenoids. The results showed that the dysfunction of most genes led to increased production of amorphadiene. The yield of amorphadiene produced by most single mutants was 8-10-fold greater compared to the wild type, indicating that theknockout sites can be engineered to promote the synthesis of exogenous terpenoids. |
| 收录类别 | SCI |
| 原文出处 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112615 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6115]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | PLOS ONE |
| 推荐引用方式 GB/T 7714 | Sun, Zhiqiang,Meng, Hailin,Li, Jing,et al. Identification of Novel Knockout Targets for Improving Terpenoids Biosynthesis inSaccharomyces cerevisiae[J]. PLOS ONE,2014. |
| APA | Sun, Zhiqiang.,Meng, Hailin.,Li, Jing.,Wang, Jianfeng.,Li, Qian.,...&Zhang, Yansheng.(2014).Identification of Novel Knockout Targets for Improving Terpenoids Biosynthesis inSaccharomyces cerevisiae.PLOS ONE. |
| MLA | Sun, Zhiqiang,et al."Identification of Novel Knockout Targets for Improving Terpenoids Biosynthesis inSaccharomyces cerevisiae".PLOS ONE (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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