Polypeptide micelles with dual pH activable dyes for sensing cells and cancer imaging
文献类型：期刊论文
| 作者 | Gong, Ping; Yang, Yueting; Yi, Huqiang; Fang, Shengtao; Zhang, Pengfei; Sheng, Zonghai; Gao, Guanhui; Gao, Duyang; Cai, Lintao |
| 刊名 | Nanoscale
 |
| 出版日期 | 2014 |
| 英文摘要 | pH is an important control parameter for maintenance of cell viability and tissue functions. pH monitoring provides valuable information on cell metabolic processes and the living environment. In this study, we prepared dual pH-sensitive, fluorescent dye-loaded polypeptide nanoparticles (DPNs) for ratiometric sensing of pHchanges in living cells. DPNs contain two types of dyes: N-(rhodamine B) lactam cystamine (RBLC), an acid activatable fluorescent dye with increased fluorescence in an acidic environment, and fluorescein isothiocyanate (FITC), a base activatable fluorescent dye with enhanced fluorescence in an alkaline environment. Hence, DPNs exhibited a dual response signal with strong red fluorescence and weak green fluorescence under acidic conditions; in contrast, they showed strong green fluorescence and almost no red fluorescence under alkaline and neutral conditions. The favorable inverse pH responses of the two fluorescent dyes resulted in ratiometric pH determination for DPNs with an optimized pH-sensitive range of pH 4.5-7.5. Quantitative analysis of the intracellular pH of intact MCF-7 cells has been successfully demonstrated with our nanosensor. Moreover, single acid activatable fluorescent dye doped polypeptide nanoparticles that only contained RBLC can distinguish tumor tissue from normal tissue by monitoring the acidic extracellular environment. This journal is © the Partner Organisations 2014.(54 refs) |
| 收录类别 | SCI |
| 原文出处 | http://pubs.rsc.org/en/Content/ArticleLanding/2014/NR/c4nr00519h#!divAbstract |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6122]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | Nanoscale |
| 推荐引用方式 GB/T 7714 | Gong, Ping,Yang, Yueting,Yi, Huqiang,et al. Polypeptide micelles with dual pH activable dyes for sensing cells and cancer imaging[J]. Nanoscale,2014. |
| APA | Gong, Ping.,Yang, Yueting.,Yi, Huqiang.,Fang, Shengtao.,Zhang, Pengfei.,...&Cai, Lintao.(2014).Polypeptide micelles with dual pH activable dyes for sensing cells and cancer imaging.Nanoscale. |
| MLA | Gong, Ping,et al."Polypeptide micelles with dual pH activable dyes for sensing cells and cancer imaging".Nanoscale (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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