Quencher-Free Fluorescent Method for Homogeneously Sensitive Detection of MicroRNAs in Human Lung Tissues
文献类型：期刊论文
| 作者 | Zhu, Guichi; Liang, Li; Zhang, Chun-yang |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014 |
| 英文摘要 | Sensitive and accurate analysis of microRNA (miRNA) expression is imperative for understanding the biological functions of miRNAs and the early diagnosis ofhuman cancers. Here, we develop a quencher-free fluorescent method for homogeneously sensitive detection of let-7a miRNA using the target-triggered recycling signal amplification in combination with a 2-aminopurine probe. The 2-aminopurine probe is characterized by the substitution of 2-aminopurine foradenine in the DNA strand and the quenching of 2-aminopurine fluorescence through its stacking interaction with the adjacent bases. The binding of target miRNA with the 2-aminopurme probe initiates the extension reaction in the presence of polymerase to produce the DNA duplexes. These DNA duplexes can be further cleaved by lambda exonuclease through the recycling digestion to release abundant free 2-arninopurines, leading to an enhanced fluorescence signal. The proposed method exhibits high sensitivity with a detection limit of 0.3 fmol, and it can even discriminate the single-base difference among the miRNA family members. More importantly, this method can accurately distinguish the expression of let-7a miRNA in human lung tissues between ten non small cell lung cancer (NSCLC) patients and ten healthy persons, holding a great potential for further application in early clinical diagnosis. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.acs.org/doi/abs/10.1021/ac503365z |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6138]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | ANALYTICAL CHEMISTRY |
| 推荐引用方式 GB/T 7714 | Zhu, Guichi,Liang, Li,Zhang, Chun-yang. Quencher-Free Fluorescent Method for Homogeneously Sensitive Detection of MicroRNAs in Human Lung Tissues[J]. ANALYTICAL CHEMISTRY,2014. |
| APA | Zhu, Guichi,Liang, Li,&Zhang, Chun-yang.(2014).Quencher-Free Fluorescent Method for Homogeneously Sensitive Detection of MicroRNAs in Human Lung Tissues.ANALYTICAL CHEMISTRY. |
| MLA | Zhu, Guichi,et al."Quencher-Free Fluorescent Method for Homogeneously Sensitive Detection of MicroRNAs in Human Lung Tissues".ANALYTICAL CHEMISTRY (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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