MicroRNA-34a inhibits the proliferation and promotes the apoptosis of non-small cell lung cancer H1299 cell line by targeting TGF beta R2
文献类型：期刊论文
| 作者 | Ma ZhongLiang; Hou PinPin; Li YanLi; Wang DeTao; Yuan TianWei; Wei JiaLi; Zhao BoTao; Lou JiaTao; Zhao XinTai; Jin Yan |
| 刊名 | TUMOR BIOLOGY
 |
| 出版日期 | 2015 |
| 英文摘要 | MicroRNAs (MiRNAs) are small non-coding RNA molecules which act as important regulators of post-transcriptional gene expression by binding 3'-untranslated region (3'-UTR) of target messenger RNA (mRNA). In this study, we analyzed miRNA-34a (miR-34a) as a tumor suppressor in non-small cell lung cancer (NSCLC) H1299 cell line. The expression level of miR-34a in four different NSCLC cell lines, H1299, A549, SPCA-1, and HCC827, was significantly lower than that in the non-tumorigenic bronchial epithelium cell line BEAS-2B. In human NSCLC tissues, miR-34a expression level was also significantly decreased in pT2-4 compared with the pT1 group. Moreover, miR-34a mimic could inhibit the proliferation and triggered apoptosis in H1299 cells. Luciferase assays revealed that miR-34a inhibited TGF beta R2 expression by targeting one binding site in the 3'-UTR of TGF beta R2 mRNA. Quantitative real-time PCR (qRT-PCR) and Western blot assays verified that miR-34a reduced TGF beta R2 expression at both mRNA and protein levels. Furthermore, downregulation of TGF beta R2 by siRNA showed the same effects on the proliferation and apoptosis as miR-34a mimic in H1299 cells. Our results demonstrated that miR-34a could inhibit the proliferation and promote the apoptosis of H1299 cells partially through the downregulation of its target gene TGF beta R2. |
| 收录类别 | SCI |
| 原文出处 | http://apps.webofknowledge.com/Search.do?product=UA&SID=V1miOCcGk5jmjeL74sA&search_mode=GeneralSearch&prID=6ee9d4c1-b24f-4156-afad-133937cafa98 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7319]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | TUMOR BIOLOGY |
| 推荐引用方式 GB/T 7714 | Ma ZhongLiang,Hou PinPin,Li YanLi,et al. MicroRNA-34a inhibits the proliferation and promotes the apoptosis of non-small cell lung cancer H1299 cell line by targeting TGF beta R2[J]. TUMOR BIOLOGY,2015. |
| APA | Ma ZhongLiang.,Hou PinPin.,Li YanLi.,Wang DeTao.,Yuan TianWei.,...&Jin YouXin.(2015).MicroRNA-34a inhibits the proliferation and promotes the apoptosis of non-small cell lung cancer H1299 cell line by targeting TGF beta R2.TUMOR BIOLOGY. |
| MLA | Ma ZhongLiang,et al."MicroRNA-34a inhibits the proliferation and promotes the apoptosis of non-small cell lung cancer H1299 cell line by targeting TGF beta R2".TUMOR BIOLOGY (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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