miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells
文献类型：期刊论文
| 作者 | Wei Jiali; Ma Zhongliang; Li Yanli; Zhao Botao; Wang Detao; Jin Yan; Jin Youxin |
| 刊名 | MOLECULAR MEDICINE REPORTS
 |
| 出版日期 | 2015 |
| 英文摘要 | microRNAs (miRNAs) are small, non-coding RNAs involved in multiple biological pathways by regulating post-transcriptional gene expression. Previously, autophagy has been reported to suppress the progression of non-small cell lung cancer (NSCLC). However, how miRNAs regulate autophagy in NSCLC remains to be elucidated. In the present study, the autophagy gene, autophagy-related 2B (ATG2B), was identified as a novel target of miR-143. The overexpression of miR-143 was able to downregulate the expression of atg2b at the transcriptional and translational levels by direct binding to its 3 untranslated region. Cell proliferation was significantly inhibited by the ectopic expression of miR-143 in H1299 cells. Knockdown of ATG2B resulted in a similar phenotype, with the overexpression of miR-143 in NSCLC cells. Furthermore, knockdown of ATG2B and hexokinase 2, a key enzyme in glycolysis and another target of miR-143, co-ordinated to inhibit the proliferation of H1299 cells. The results of the present study demonstrated that miR-143 was a novel and important regulator of autophagy by targeting ATG2B and repression of gene expression in autophagy and high glycolysis had a coordinate effect in H1299 cells. These results suggested that ATG2B may be a new potential therapeutic target for NSCLC. Furthermore, it was implied that interrupting autophagy and glycolysis improves NSCLC therapy. |
| 收录类别 | SCI |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pubmed/25322940 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7320]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | MOLECULAR MEDICINE REPORTS |
| 推荐引用方式 GB/T 7714 | Wei Jiali,Ma Zhongliang,Li Yanli,et al. miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells[J]. MOLECULAR MEDICINE REPORTS,2015. |
| APA | Wei Jiali.,Ma Zhongliang.,Li Yanli.,Zhao Botao.,Wang Detao.,...&Jin Youxin.(2015).miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells.MOLECULAR MEDICINE REPORTS. |
| MLA | Wei Jiali,et al."miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells".MOLECULAR MEDICINE REPORTS (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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