N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies
文献类型：期刊论文
| 作者 | Dezhi Li; Jie Liu; Li Zhang; Tianshu Xu; Junheng Chen; Liping Wang; Qi Zhao |
| 刊名 | VIROLOGICA SINICA
 |
| 出版日期 | 2015 |
| 英文摘要 | The Human immunodeficiency virus type 1 (HIV-1) gp41 membrane proximal external region (MPER) is targeted by broadly neutralizing antibodies (e.g. 2F5, 4E10, Z13e and m66.6), which makes this region a promising target for vaccine design. One strategy to elicit neutralizing antibodies against the MPER epitope is to design peptide immunogens mimicking neutralization structures. To probe 2F5-like neutralizing antibodies, two yeast-displayed antibody libraries from peripheral blood mononuclear cells from a HIV-1 patient were screened against the 2F5 epitope peptide SP62. Two 2F5-like antibodies were identified that specifically recognized SP62. However, these antibodies only weakly neutralized HIV-1 primary isolates. The epitopes recognized by these two 2F5-like antibodies include not only the 2F5 epitope (amino acids (aa) 662–667 in the MPER) but also several other residues (aa 652–655) locating at the N-terminus in SP62. Experimental results suggest that residues of SP62 adjacent to the 2F5 epitope influence the response of broadly neutralizing 2F5-like antibodies in vaccination. Our findings may aid the design of vaccine immunogens and development of therapeutics against HIV-1 infection. |
| 收录类别 | SCI |
| 原文出处 | http://www.virosin.org/Paper/2015/30/06/VS12250-015-3664-6 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7335]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | VIROLOGICA SINICA |
| 推荐引用方式 GB/T 7714 | Dezhi Li,Jie Liu,Li Zhang,et al. N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies[J]. VIROLOGICA SINICA,2015. |
| APA | Dezhi Li.,Jie Liu.,Li Zhang.,Tianshu Xu.,Junheng Chen.,...&Qi Zhao.(2015).N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies.VIROLOGICA SINICA. |
| MLA | Dezhi Li,et al."N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies".VIROLOGICA SINICA (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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