A single quantum dot-based biosensor for DNA point mutation assay
文献类型：期刊论文
| 作者 | Wei Tang; Guichi Zhu; Li Liang; Chun-yang Zhang |
| 刊名 | ANALYST
 |
| 出版日期 | 2015 |
| 英文摘要 | we demonstrate a single quantum dot (QD)-based biosensor for sensitive DNA point mutation assay. In this assay, the mutant target (G/C) remains unchanged after the endonuclease treatment, and the polymerase chain reaction (PCR) may be initiated with the assistance of primers and polymerase, generating a large number of mutant targets. The amplified mutant targets can be captured by the biotinylated probes during the process of denaturation and annealing, and the Cy5-dGTP may be assembled into the biotinylated probe with the catalysis of polymerase, leading to the formation of Cy5-labeled biotinylated probes. The Cy5-labeled biotinylated probes can be further assembled onto the QD surface to obtain a Cy5-DNA-QD complex, resulting in the generation of fluorescence resonance energy transfer (FRET) between the QD donor and the Cy5 receptor. The mutant targets can be quantitatively evaluated through the measurement of Cy5 counts by total internal reflection fluorescence (TIRF) microscopy. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.rsc.org/en/Content/ArticleLanding/2015/AN/C5AN01270H#!divAbstract |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7368]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | ANALYST |
| 推荐引用方式 GB/T 7714 | Wei Tang,Guichi Zhu,Li Liang,et al. A single quantum dot-based biosensor for DNA point mutation assay[J]. ANALYST,2015. |
| APA | Wei Tang,Guichi Zhu,Li Liang,&Chun-yang Zhang.(2015).A single quantum dot-based biosensor for DNA point mutation assay.ANALYST. |
| MLA | Wei Tang,et al."A single quantum dot-based biosensor for DNA point mutation assay".ANALYST (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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