LKB1 Is Required for the Development and Maintenance of Stereocilia in Inner Ear Hair Cells in Mice
文献类型：期刊论文
| 作者 | Yuqin Men; Aizhen Zhang; Haixiang Li; Tingting Zhang; Yecheng Jin; Huashun Li; Jian Zhang; Jiangang Gao |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015 |
| 英文摘要 | The LKB1 gene, which encodes a serine/threonine kinase, was discovered to play crucial roles in cell differentiation, proliferation, and the establishmentof cell polarity. In our study, LKB1 conditional knockout mice (Atoh1-LKB1(-/-) mice) were generated to investigate LKB1 function in the inner ear. Testsof auditory brainstem response and distortion product otoacoustic emissions revealed significant decreases in the hearing sensitivities of the Atoh1-LKB1(-/-) mice. In Atoh1-LKB1(-/-) mice, malformations of hair cell stereocilliary bundles were present as early as postnatal day 1 (P1), a time long before the maturation of the hair cell bundles. In addition, we also observed outer hair cell (OHC) loss starting at P14. The impaired stereocilliary bundles occurred long before the presence of hair cell loss. Stereociliary cytoskeletal structure depends on the core actin-based cytoskeleton and several actin-binding proteins. By Western blot, we examined actin-binding proteins, specifically ERM (ezrin/radixin/moesin) proteins involved in the regulation of theactin cytoskeleton of hair cell stereocilia. Our results revealed that the phosphorylation of ERM proteins (pERM) was significantly decreased in mutantmice. Thus, we propose that the decreased pERM may be a key factor for the impaired stereocillia function, and the damaged stereocillia may inducehair cell loss and hearing impairments. Taken together, our data indicates that LKB1 is required for the development and maintenance of stereocilia inthe inner ear. |
| 收录类别 | SCI |
| 原文出处 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135841 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7371]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | PLOS ONE |
| 推荐引用方式 GB/T 7714 | Yuqin Men,Aizhen Zhang,Haixiang Li,et al. LKB1 Is Required for the Development and Maintenance of Stereocilia in Inner Ear Hair Cells in Mice[J]. PLOS ONE,2015. |
| APA | Yuqin Men.,Aizhen Zhang.,Haixiang Li.,Tingting Zhang.,Yecheng Jin.,...&Jiangang Gao.(2015).LKB1 Is Required for the Development and Maintenance of Stereocilia in Inner Ear Hair Cells in Mice.PLOS ONE. |
| MLA | Yuqin Men,et al."LKB1 Is Required for the Development and Maintenance of Stereocilia in Inner Ear Hair Cells in Mice".PLOS ONE (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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