LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration
文献类型：期刊论文
| 作者 | Yuqin Men; Aizhen Zhang; Haixiang Li; Yecheng Jin; Xiaoyang Sun; Huashun Li; Jiangang Gao |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 英文摘要 | The Liver Kinase B1 (LKB1) gene plays crucial roles in cell differentiation, proliferation and the establishment of cell polarity. We created LKB1conditional knockout mice (LKB1(Atoh1) CKO) to investigate the function of LKB1 in cerebellar development. The LKB1Atoh1 CKO mice displayed motor dysfunction. In the LKB1Atoh1 CKO cerebellum, the overall structure had a larger volume and morelobules. LKB1 inactivationled to an increasedproliferation of granule cell precursors (GCPs), aberrant granule cell migration and overproduction of unipolar brush cells. To investigate the mechanism underlying the abnormal foliation, we examined sonic hedgehog signalling (Shh) by testing its transcriptional mediators, the Gli proteins, which regulate the GCPs proliferation and cerebellar foliation during cerebellar development. The expression levels of Gli genes were significantly increased in the mutant cerebellum. In vitro assays showed that the proliferation of cultured GCPs from mutant cerebellum significantly increased, whereas theproliferation of mutant GCPs significantly decreased in the presence of a Shh inhibitor GDC-0049. Thus, LKB1 deficiency in the LKB1(Atoh1) CKO mice enhanced Shh signalling, leading to the excessive GCP proliferation and the formation of extra lobules. We proposed that LKB1 regulates cerebellardevelopment by controlling GCPs proliferation through Shh signalling during cerebellar development. |
| 收录类别 | SCI |
| 原文出处 | http://www.nature.com/articles/srep16232 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7372]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | SCIENTIFIC REPORTS |
| 推荐引用方式 GB/T 7714 | Yuqin Men,Aizhen Zhang,Haixiang Li,et al. LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration[J]. SCIENTIFIC REPORTS,2015. |
| APA | Yuqin Men.,Aizhen Zhang.,Haixiang Li.,Yecheng Jin.,Xiaoyang Sun.,...&Jiangang Gao.(2015).LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration.SCIENTIFIC REPORTS. |
| MLA | Yuqin Men,et al."LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration".SCIENTIFIC REPORTS (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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