Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition
文献类型：期刊论文
| 作者 | Cui J; Jin G; Yu B; Wang Z; Lin R; Huang JD. |
| 刊名 | Biochemical and Biophysical Research Communications
 |
| 出版日期 | 2015 |
| 英文摘要 | Polarization of epithelial cells requires vectorial sorting and transport of polarity proteins to apical or basolateral domains. Kif5b is the mouse homologue of the human ubiquitous Kinesin Heavy Chain (uKHC). To investigate the function of Kif5b in epithelial cells, we examined the phenotypes of Kif5b-deficient MDCK cells. Stable knockdown of Kif5b in MDCK cells resulted in reduced cell proliferation rate, profound changes in cell morphology, loss of epithelial cell marker, and gain of mesenchymal marker, as well as increased cell migration, invasion, and tumorigenesis abilities. E-cadherin and NMMIIA could interact with Kif5b in polarized MDCK cells, and their expression levels were decreased in Kif5b-deficient MDCK cells. Overexpression of E-cadherin and NMMIIA in Kif5b depleted MDCK cells could decrease mesenchymal marker expression and cell migration ability. These results indicate that stable knockdown of Kif5b in MDCK cells can lead to epithelial-mesenchymal transition, which is mediated by defective E-cadherin and NMMIIA expression. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0006291X15009614 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7393]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | Biochemical and Biophysical Research Communications |
| 推荐引用方式 GB/T 7714 | Cui J,Jin G,Yu B,et al. Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition[J]. Biochemical and Biophysical Research Communications,2015. |
| APA | Cui J,Jin G,Yu B,Wang Z,Lin R,&Huang JD..(2015).Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition.Biochemical and Biophysical Research Communications. |
| MLA | Cui J,et al."Stable knockdown of Kif5b in MDCK cells leads to epithelial-mesenchymal transition".Biochemical and Biophysical Research Communications (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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