In situ crosslinked smart polypeptide nanoparticles for multistage responsive tumor-targeted drug delivery
文献类型：期刊论文
| 作者 | Yi, Huqiang; Liu, Peng; Sheng, Nan; Gong, Ping; Ma, Yifan; Cai, Lintao |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2016 |
| 英文摘要 | Smart tumor-targeted drug delivery is crucial for improving the effect of chemotherapy and reducing the adverse effects. Here, we synthesized a smart polypeptide copolymer based on n-butylamine-poly(Llysine)- b-poly(L-cysteine) (PLL-PLC) with functionalization of folic acid (FA) and 1,2-dicarboxylic-cyclohexene anhydride (DCA) for multistage responsive tumor-targeted drug delivery. The copolymers (FA-PLL( DCA)-PLC) spontaneously crosslinked in situ to form redox and pH dual responsive FA-PLL(DCA)-PLCnanoparticles (FD-NPs), which had a reversible zeta potential around -30 mV at pH 7.4, but switched to + 15 mV at pH 5.0. Moreover, FD-NPs effectively loaded DOX with a loading capacity at 15.7 wt%. At pH 7.4, only 24.5% DOX was released within 60 h. However, at pH 5.0, the presence of 10 mM DTT dramatically accelerated DOX release with over 90% of DOX released within 10 h. Although the FD-NPs only enhanced DOX uptake in FA receptor positive (FR+) cancer cells at pH 7.4, a weak acidic condition promoted FD-NP-facilitated DOX uptake in both FR+ HeLa and FR-A549 cells, as well as significantly improving cellular binding and end/lysosomal escape. In vivo studies in a HeLa cancer model demonstrated that the charge-reversible FD-NPs delivered DOX into tumors more effectively than charge-irreversible nanoparticles. Hence, these multistage responsive FD-NPs would serve as highly efficient drug vectors for targeted cancer chemotherapy. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.rsc.org/en/content/articlehtml/2016/nr/c5nr07348k |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10660]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | NANOSCALE |
| 推荐引用方式 GB/T 7714 | Yi, Huqiang,Liu, Peng,Sheng, Nan,et al. In situ crosslinked smart polypeptide nanoparticles for multistage responsive tumor-targeted drug delivery[J]. NANOSCALE,2016. |
| APA | Yi, Huqiang,Liu, Peng,Sheng, Nan,Gong, Ping,Ma, Yifan,&Cai, Lintao.(2016).In situ crosslinked smart polypeptide nanoparticles for multistage responsive tumor-targeted drug delivery.NANOSCALE. |
| MLA | Yi, Huqiang,et al."In situ crosslinked smart polypeptide nanoparticles for multistage responsive tumor-targeted drug delivery".NANOSCALE (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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