Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b
文献类型：期刊论文
| 作者 | Shao, Ximing; Liu, Yi; Yu, Qian; Ding, Zhihao; Qian, Wenyu; Zhang, Lei; Zhang, Jianchao; Jiang, Nan; Gui, Linfei; Xu, Zhiheng |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2016 |
| 英文摘要 | Numb is an endocytic protein that plays crucial roles in diverse cellular processes such as asymmetric cell division, cell migration and differentiation. However, the molecular mechanism by which Numb regulatesendocytic trafficking is poorly understood. Here, we demonstrate that Numb is a docking regulator forhomotypic fusion of early endosomes (EEs). Numb depletion causes clustered but unfused EEs, which can be rescued by overexpressing cytosolic Numb 65 and Numb 71 but not plasma membrane-attached Numb66 or Numb 72. Time-lapse analysis reveals that paired vesicles tend to tether but not fuse with each other in the absence of Numb. We further show that Numb binds to another docking regulator, Mon1b, and is required for the recruitment of cytosolic Mon1b to the EE membrane. Consistent with this, deletion ofMon1b causes similar defects in EE fusion. Our study thus identifies a novel mechanism by which Numbregulates endocytic sorting by mediating EE fusion. |
| 收录类别 | SCI |
| 原文出处 | http://www.nature.com/cr/journal/v26/n5/full/cr201634a.html |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10686]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | CELL RESEARCH |
| 推荐引用方式 GB/T 7714 | Shao, Ximing,Liu, Yi,Yu, Qian,et al. Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b[J]. CELL RESEARCH,2016. |
| APA | Shao, Ximing.,Liu, Yi.,Yu, Qian.,Ding, Zhihao.,Qian, Wenyu.,...&Li, Huashun.(2016).Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.CELL RESEARCH. |
| MLA | Shao, Ximing,et al."Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b".CELL RESEARCH (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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