The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely
文献类型：期刊论文
| 作者 | Guo, Xiaoyan; Chen, Ping; Hou, Xiaohu; Xu, Wenjuan; Wang, Dan; Wang, Tian-yan; Zhang, Liping; Zheng, Gang; Gao, Zhi-liang; He, Cheng-Yi |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 英文摘要 | HBV covalently closed circular DNA (cccDNA) is drug-resistant and responsible for viral persistence. To facilitate the development of anti-cccDNA drugs, we developed a minicircle DNA vector (MC)-basedtechnology to produce large quantity of recombined cccDNA (rcccDNA) resembling closely to its wild-type counterpart both in structure and function. The rcccDNA differed to the wild-type cccDNA (wtcccDNA) onlyin that it carried an extra 36-bp DNA recombinant product attR upstream of the preC/C gene. Using a procedure similar to standard plasmid production, milligrams of rcccDNA can be generated in common laboratories conveniently. The rcccDNA demonstrated many essential biological features of wtcccDNA, including: (1) undergoing nucleation upon nucleus entry; (2) serving as template for production of all HBVRNAs and proteins; (3) deriving virions capable of infecting tree shrew, and subsequently producing viral mRNAs, proteins, rcccDNA and infectious virions. As an example to develop anti-cccDNA drugs, we usedthe Crispr/Cas9 system to provide clear-cut evidence that rcccDNA was cleaved by this DNA editing tool invitro. In summary, we have developed a convenient technology to produce large quantity of rcccDNA as a surrogate of wtcccDNA for investigating HBV biology and developing treatment to eradicate this most wide-spreading virus. |
| 收录类别 | SCI |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865889/ |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10717]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | SCIENTIFIC REPORTS |
| 推荐引用方式 GB/T 7714 | Guo, Xiaoyan,Chen, Ping,Hou, Xiaohu,et al. The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely[J]. SCIENTIFIC REPORTS,2016. |
| APA | Guo, Xiaoyan.,Chen, Ping.,Hou, Xiaohu.,Xu, Wenjuan.,Wang, Dan.,...&Chen, Zhi-Ying.(2016).The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely.SCIENTIFIC REPORTS. |
| MLA | Guo, Xiaoyan,et al."The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely".SCIENTIFIC REPORTS (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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