A Bone-Implant Interaction Mouse Model for Evaluating Molecular Mechanism of Biomaterials/Bone Interaction.
文献类型：期刊论文
| 作者 | Liu Wenlong; Dan Xiuli; Wang Ting; Lu William W.; Pan Haobo |
| 刊名 | TISSUE ENGINEERING PART C-METHODS
 |
| 出版日期 | 2016 |
| 英文摘要 | The development of an optimal animal model which could provide fast assessments of the interaction between bone and orthopaedic implants is essential for both pre-clinical and theoretical researches in the design of novel biomaterials. Compared with other animal models, mice have superiority in accessing the well-developed transgenic modification techniques (e.g. cell tracing, knock-off, knock-in ect.) which serve as powerful tools in studying molecular mechanisms. In this study, we introduced the establishment of a mouse model which is specifically tailored for the assessment of bone-implant interaction in a load-bearing bone marrow microenvironment and could potentially allow the molecular mechanism study of biomaterials by using transgenic technologies. The detailed microsurgery procedures for developing a bone defect (Φ=0.8 mm) at the metaphysis region of the mouse femur were recorded. According to our results, the osteoconductive and osseointegrative properties of a well-studied 45S5 bioactive glass were confirmed by utilizing our mouse model, verifying the reliability of this model. The feasibility and reliability of the present model were further checked by using other materials as objects of study. Furthermore, this animal model provided a more homogenous tissue-implant interacting surface than the rat at the early stage of implantation and this is quite meaningful for conducting quantitatively analysis. The availability of transgenic techniques to mechanism study of biomaterials was further testified by establishing our model on Nestin-GFP transgenic mice. Intriguingly, the distribution of nestin+ cells was demonstrated to be recruited to the surface of 45S5 glass as early as 3 days post-surgery, indicating that nestin+ lineage stem cells may participate in the subsequent regeneration process. In summary, the bone-implant interaction mouse model could serve as a potential candidate to evaluate the early-stage tissue response near the implant surface in a bone marrow microenvironment; and it also shows great potentials in making transgenic animal resource applicable to biomaterial studies, so that the design of novel biomaterials could be better guided. |
| 收录类别 | SCI |
| 原文出处 | https://www.ncbi.nlm.nih.gov/pubmed/27733092 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10777]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | TISSUE ENGINEERING PART C-METHODS |
| 推荐引用方式 GB/T 7714 | Liu Wenlong,Dan Xiuli,Wang Ting,et al. A Bone-Implant Interaction Mouse Model for Evaluating Molecular Mechanism of Biomaterials/Bone Interaction.[J]. TISSUE ENGINEERING PART C-METHODS,2016. |
| APA | Liu Wenlong,Dan Xiuli,Wang Ting,Lu William W.,&Pan Haobo.(2016).A Bone-Implant Interaction Mouse Model for Evaluating Molecular Mechanism of Biomaterials/Bone Interaction..TISSUE ENGINEERING PART C-METHODS. |
| MLA | Liu Wenlong,et al."A Bone-Implant Interaction Mouse Model for Evaluating Molecular Mechanism of Biomaterials/Bone Interaction.".TISSUE ENGINEERING PART C-METHODS (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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