Controllable Mismatched Ligation for Bioluminescence Screening of Known and Unknown Mutations
文献类型：期刊论文
| 作者 | Xu, Qinfeng; Huang, Si-qiang; Ma, Fei; Tang, Bo; Zhang, Chun-yang |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 英文摘要 | Single-nucleotide polymorphisms (SNPs) are closely related to human diseases and individual drug responses, and the accurate detection of SNPs is crucial to both clinical diagnosis and development ofpersonalized medicine. Among various SNPs detection methods, ligase detection reaction (LDR) has shown great potential due to its low detection limit and excellent specificity. However, frequent involvement ofexpensive labels increases the experimental cost and compromises the assay efficiency, and the requirement of careful predesigned probes limits it to only known SNPs assays. In this research, we develop a controllable mismatched ligation for bioluminescence screening of both known and unknown mutations. Especially, the ligation specificity of E. coli ligase is tunable under different experimental conditions. The mismatches locating on the 3'-side of the nick cannot be ligated efficiently by E. coli ligase, whereas all mismatches locating on the 5'-side of the nick can be ligated efficiently by E. coli ligase. We design a 3'-discriminating probe (3'-probe) for the discrimination of known mutation and introduce a T7 Endo I for the detection of unknown mutation. With the integration of bioluminescence monitoring of ligation byproduct adenosine S'-monophosphate (AMP), both known and unknown SNPs can be easily detected without the involvement of any expensive labels and labor-intensive separation. This method is simple, homogeneous, label-free, and cost-effective and may provide a valuable complement to current sequencing technologies fordisease diagnostics, personalized medicine, and biomedical research. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04540 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10798]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | ANALYTICAL CHEMISTRY |
| 推荐引用方式 GB/T 7714 | Xu, Qinfeng,Huang, Si-qiang,Ma, Fei,et al. Controllable Mismatched Ligation for Bioluminescence Screening of Known and Unknown Mutations[J]. ANALYTICAL CHEMISTRY,2016. |
| APA | Xu, Qinfeng,Huang, Si-qiang,Ma, Fei,Tang, Bo,&Zhang, Chun-yang.(2016).Controllable Mismatched Ligation for Bioluminescence Screening of Known and Unknown Mutations.ANALYTICAL CHEMISTRY. |
| MLA | Xu, Qinfeng,et al."Controllable Mismatched Ligation for Bioluminescence Screening of Known and Unknown Mutations".ANALYTICAL CHEMISTRY (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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