Multicolor Quantum Dot-Based Chemical Nose for Rapid and Array-Free Differentiation of Multiple Proteins
文献类型：期刊论文
| 作者 | Xu, Qinfeng; Zhang, Yihong; Tang, Bo; Zhang, Chun-yang |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 英文摘要 | Nanomaterial-based differential sensors (e.g., chemical nose) have shown great potential for identification ofmultiple proteins because of their modulatable recognition and transduction capability but with the limitationof array separation, single-channel read-out, and long incubation time. Here, we develop a multicolorquantum dot (QD)-based multichannel sensing platform for rapid identification of multiple proteins in anarray-free format within 1 min. A protein-binding dye of bromophenol blue (BPB) is explored as an efficient reversible quencher of QDs, and the mixture of BPB with multicolor QDs may generate the quenched QD-BPB complexes. The addition of proteins will disrupt the QD-BPB complexes as a result of the competitive protein-BPB binding, inducing the separation of BPB from the QDs and the generation of distinct fluorescence patterns. The multicolor patterns may be collected at a single-wavelength excitation and differentiated by a linear discriminant analysis (LDA). This multichannel sensing platform allows for the discrimination of ten proteins and seven cell lines with the fastest response rate reported to date, holding great promise for rapid and high-throughput medical diagnostics. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b03109 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10799]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | ANALYTICAL CHEMISTRY |
| 推荐引用方式 GB/T 7714 | Xu, Qinfeng,Zhang, Yihong,Tang, Bo,et al. Multicolor Quantum Dot-Based Chemical Nose for Rapid and Array-Free Differentiation of Multiple Proteins[J]. ANALYTICAL CHEMISTRY,2016. |
| APA | Xu, Qinfeng,Zhang, Yihong,Tang, Bo,&Zhang, Chun-yang.(2016).Multicolor Quantum Dot-Based Chemical Nose for Rapid and Array-Free Differentiation of Multiple Proteins.ANALYTICAL CHEMISTRY. |
| MLA | Xu, Qinfeng,et al."Multicolor Quantum Dot-Based Chemical Nose for Rapid and Array-Free Differentiation of Multiple Proteins".ANALYTICAL CHEMISTRY (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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