Polypeptide Micelles Delivered Suicide Gene Effectively Inhibited Hepatoma Cancer Cells Proliferation
文献类型：会议论文
| 作者 | Cuifang Zheng; Huqiang Yi; Shengtao Fang; Guolin Li; Ping Gong; Mingbin Zheng; Pengfei Zhang; Lintao Cai |
| 出版日期 | 2015 |
| 会议名称 | ChinaNanomedicine 2015 |
| 会议地点 | Hangzhou, China |
| 英文摘要 | Gene therapy can be defined as the transfer of genetic material into a cell for therapeutic purposes. Cytosine deaminase (CD) transferred into tumor cells by an adenoviral vector, can convert the antifungal drug fluorocytosine (5-FC) to the antimetabolite 5-fluorouracil (5-FU), which kills not only the transfected tumor cells but also their neighbors by the so-called‘bystander effect’. Here, we prepared polypeptide micelle nanoparticles (NP) of a triblock copolymer poly(ethylene glycol)-b-poly(L-lysine)-b-poly(L-leucine) (PEG-PLL- PLLeu) to systemically deliver CD plasmid for an effective gene vector. The promoter sequences of the gene that are highly active in hepatoma tumor cells, HE4, was used to target HepG2 tumor cells. We constructed the CD plasmid and successfully transfected into HepG2 tumor cells. The transfection efficiency of CD suicide gene complexes in HepeG2 cells was 73.1% . This suicid gene delivery system can significantly inhibited HepeG2 tumor cell Proliferation. Our results demonstrated well-defined PEG-PLL-PLLeu polypeptide cationic micelles with the excellent gene delivery of CD plasmid and 5-FC in combined cancer therapy, which holds promise as an effective therapy for advanced-stage hepatoma cancer. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| WOS记录号 | WOS:000373923400278 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7442]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | 2015 |
| 推荐引用方式 GB/T 7714 | Cuifang Zheng,Huqiang Yi,Shengtao Fang,et al. Polypeptide Micelles Delivered Suicide Gene Effectively Inhibited Hepatoma Cancer Cells Proliferation[C]. 见:ChinaNanomedicine 2015. Hangzhou, China. |
入库方式： OAI收割
来源：深圳先进技术研究院
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