"Off-On" Switchable Sensor: a Near-Infrared Fluorescent pH Probe and Its Imaging Applications in Vivo
文献类型：会议论文
| 作者 | Xiaoqing Meng; Yalin Lv; Li Zhang; Ping Gong; Lintao Cai |
| 出版日期 | 2015 |
| 会议名称 | ChinaNanomedicine 2015 |
| 会议地点 | Hangzhou, China |
| 英文摘要 | Intracellular or extracellular pH is influenced by diverse physiological and pathological processes, so quantitatively measuring pH is useful for cellular analysis or diagnosis. In this work, a new near-infrared (NIR) fluorescence turn-on probe CP utilizing a fluorophore-spacer- receptor molecular framework that can modulate the fluorescence emission intensity through a fast photoinduced electron-transfer process was developed. Our strategy was to choose tricarbocyanine (Cy), a NIR fluorescent dye with high extinction coefficients, as a fluorophore, and N-(4-Pyridylmethyl)ethylenediamine (Py) as a receptor. The pH titration indicated that CP can monitor the minor physiological pH fluctuations with a pKa of ∼6.40, which is valuable for intracellular pH researches and tumor diagnosis. The probe responds linearly and rapidly to minor pH fluctuations within the range of 5.00-7.50 and exhibits strong dependence on pH changes. As expected, the real-time imaging of cellular pH and the detection of pH in situ was achieved successfully in living A549 and HeLa cells by this probe. It is shown that the probe effectively avoids the influence of autofluorescence and native cellular species in biological systems and meanwhile exhibits high sensitivity, good photostability, low cytotoxicity and excellent cell membrane permeability. This fluorescence probe is further examined as tumor microenvironment activatable probes for intravenous in vivo tumor imaging, which is expected to be of crucial importance for clinical tumor diagnostics. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| WOS记录号 | WOS:000373923400256 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7446]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | 2015 |
| 推荐引用方式 GB/T 7714 | Xiaoqing Meng,Yalin Lv,Li Zhang,et al. "Off-On" Switchable Sensor: a Near-Infrared Fluorescent pH Probe and Its Imaging Applications in Vivo[C]. 见:ChinaNanomedicine 2015. Hangzhou, China. |
入库方式： OAI收割
来源：深圳先进技术研究院
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