Chemerin secreted from gonadal adipose tissue promotes ovarian cancer progression through the CMKLR1 signaling pathway
文献类型：会议论文
| 作者 | Xiao-yun Qin; Xiao-fei Tian; Ye Zhang; Ya-li Yang; Jie Chen; Tian-Xia Xiao; Brian A. Zabel; Hui -Ru Tang; Li-Kuan Xiong; Pei-Gen Ren |
| 出版日期 | 2016 |
| 会议名称 | The 4th SKLRB Symposium on Reproductive Biology |
| 会议地点 | 中国北京 |
| 英文摘要 | Ovarian cancer is one of the most diagnosed types of cancer in women. Studies have indicated that obesity and chemokines play key roles in ovarian cancer, but whether chemerin secreted by gonadal white adipose tissue (gWAT) affects the progress of ovarian cancer through the activation of one of its receptors – CMKLR1 – remains unknown. To investigate this question, we characterized the effects of suppressing CMKLR1 function on cancer-related properties and gene expression in the ovarian cancer cell line SKOV3 by several assays, including measurement of cell proliferation, invasion, colony formation, and tumorigenicity in mice, and western blotting and Q-PCR to quantify gene and protein expression. CMKLR1 expression was knocked down using short hairpin RNA silencing, and CMKLR1 protein function was blocked using the CMKLR1 antagonist α-NETA. CMKLR1 gene silencing attenuated the expression of MMP-2, uPA, MUC16, VEGF, and MMP9 and decreased cell migration and invasion, while chemerin had the opposite effects. These results were confirmed by results obtained in vivo. We also found that both α-NETA and CMKLR1 shRNA suppressed tumorigenesis in mice and that the tumors tended to migrate to regions of higher chemerin concentration. Our data show that CMKLR1 silencing leads to the inhibition of proliferation, migration, and invasion by SKOV3 cells, whereas chemerin reverses this inhibition. The data also suggest that the migration of tumors that form in the mouse xenograft model depends on the CMKLR1 pathway. To our knowledge, this is the first report on a role for CMKLR1 in ovarian cancer formation and invasion. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10824]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | 2016 |
| 推荐引用方式 GB/T 7714 | Xiao-yun Qin,Xiao-fei Tian,Ye Zhang,et al. Chemerin secreted from gonadal adipose tissue promotes ovarian cancer progression through the CMKLR1 signaling pathway[C]. 见:The 4th SKLRB Symposium on Reproductive Biology. 中国北京. |
入库方式： OAI收割
来源：深圳先进技术研究院
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