Abnormal placental development in CMKLR1 deficient mice
文献类型：会议论文
| 作者 | Xiujun Fan; Qingqing Zhang; Zhonglin Xiao; Liang Xiang; Brian A Zabel; Qing Yang; Jian V Zhang |
| 出版日期 | 2016 |
| 会议名称 | The Human Placenta Project (HPP): Understanding Human Placental Structure and Function in Real Time 3rd Annual Meeting |
| 会议地点 | Bethesda, MD |
| 英文摘要 | OBJECTIVES: Adipokine chemerin was abnormally expressed in pregnancy complications, such as preeclampsia etc.. But, the role of chemerin and its receptors in normal pregnancy and disease conditions is not clear. To address this issue, pregnancy outcome and placental development in CMKLR1, the native receptor of chemerin, deficient mouse were investigated. METHODS: Uteri, implantation sites and placentas were collected on gestation day (GD) 1, 5, 8 and 12 from CMKLR1-/- and wild type (WT) mice. The mRNA level of chemerin and its receptors, MMPs and TIMPs were measured by RT-PCR. Placenta morphology was evaluated on H&E stained sections. Changes of trophoblasts, uNK cells and vasculature system in placenta were evaluated by cytokeratin 8 (CK8), DBA lectin and isolectin BS1 immunohistohemistry (IHC) separately. RESULTS: Chemerin mRNA is expressed in placenta with peak level at GD12 in WT mouse on examed samples. There is no differences in pregnancy outcomes and chemerin levels between CMKLR1-/- and WT. However, at GD12, the labyrinth layer is thicker, the maternal blood space is increased in the labyrinth layer, the trophoblast cells invaded deeper into the deciduas and the spiral arteries at the junction zone in CMKLR1-/- compared with WT. In addition, we found the expression of MMP2 mRNA was increased significantly with no changes of MMP9, TIMP1 and TIMP2, and the number of uNK cells was significantly increased at the implantation sites of CMKLR1-/- mice. CONCLUSIONS: These results imply that chemerin might involve with trophoblast invasion and vasculogenesis in placenta by regulating MMP2 and uNK cells through CMKLR1 receptor. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10827]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | 2016 |
| 推荐引用方式 GB/T 7714 | Xiujun Fan,Qingqing Zhang,Zhonglin Xiao,et al. Abnormal placental development in CMKLR1 deficient mice[C]. 见:The Human Placenta Project (HPP): Understanding Human Placental Structure and Function in Real Time 3rd Annual Meeting. Bethesda, MD. |
入库方式： OAI收割
来源：深圳先进技术研究院
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