VEGF-165 Regulated sFlt-1 Production in Tropholbast through Both Flt-1 and KDR Receptors
文献类型：会议论文
| 作者 | Zhonglin Xiao; Mingtao Xu; Lijiang Ge; Qing Yang; Xiujun Fan |
| 出版日期 | 2016 |
| 会议名称 | The 4th SKLRB Symposium on Reproductive Biology |
| 会议地点 | 中国北京 |
| 英文摘要 | Preeclampsia (PE) is a severe pregnancy complication characterized by hypertension, proteinuria, edema after 20 weeks of gestation in pregnant women. And it accounts for 14% of pregnancy-related maternal death and 15% of preterm birth. To date, the mechanism of this disease development is still unclear. Recently, study showed that excess deciduas origin VEGF stimulated sFlt-1 over-production in the trophoblast is one of the causes for PE. However, how VEGF regulates sFtl-1 expression in the trophoblasts is still unknown. To address this issue, tropholbast cell lines were used to investigate the signal pathways involved with the regulation of sFlt-1 by VEGF in vitro. VEGF overexpressing JEG3 (VEGF-GFP-JEG3) and HTR8/SV neo (VEGF-GFP-HTR8) cell lines were established by infecting JEG3 and HTR8/SV neo cell lines with lentiviruses expressing VEGF-165. Then, the sFlt-1 expression, migration and invasion functional changes in these cell lines were compared with JEG3 and HTR8/SV neo in the following treatment groups: control group, Flt-1 receptor inhibitor (MK-2461) group, KDR receptor inhibitor (ABT869) group, and Flt-1 and KDR receptors inhibitor (XL-184) group. The mRNA and protein level of sFlt-1 was dramatically upregulated in the VEGF-GFP-JEG3 compared with JEG3 cells, and was decreased significantly when they were treated by MK-2461, ABT-869 and XL-184 respectively. And the migration rate of these three groups of cells was significantly increased compared with VEGF-GFP-JEG3. Interestingly, the mRNA level of VEGF-A, sFlt-1, Flt-1, and KDR in VEGF-GFP-HTR8 cells are all increased significantly in the cells after the treatment of MK-2461, ABT-869 and XL-184. But the protein level of sFlt-1 turned out decreased significantly. In addition, the migration rate of these three groups of cells was significantly increased compared with VEGF-GFP-HTR8. These results indicate that sFlt-1 up-regulation by VEGF through the VEGF/Flt-1 and/or the VEGF/KDR signaling pathway, but which one is playing the key roles needs further investigation. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10828]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | 2016 |
| 推荐引用方式 GB/T 7714 | Zhonglin Xiao,Mingtao Xu,Lijiang Ge,et al. VEGF-165 Regulated sFlt-1 Production in Tropholbast through Both Flt-1 and KDR Receptors[C]. 见:The 4th SKLRB Symposium on Reproductive Biology. 中国北京. |
入库方式： OAI收割
来源：深圳先进技术研究院
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