Medical Abortion by Trophoblast-Targeted Nanopartciles Loaded with methotrexate
文献类型：会议论文
| 作者 | Baozhen Zhang; Lunbo Tan; Baobei Wang; Jie Chen; Mengxia Li; Tianxia Xiao; Qing Yang; Xiujun Fan |
| 出版日期 | 2016 |
| 会议名称 | The 4th SKLRB Symposium on Reproductive Biology |
| 会议地点 | 中国北京 |
| 英文摘要 | Medical abortion (MA) is an increasingly preferred option for pregnancy termination in early gestation in many countries. Several drug regimens are used with varying efficacy including mifepristone (MIFE), misoprostol (MISO) and methotrexate (MTX), but they all have some side effects. Here we report a technology potentially overcoming these shortcomings and enhancing their efficacy through a trophoblast targeted nanoparticle (pl-CSA-MNPs) made by using a placenta chondroitin sulfate A ( pl-CSA) binding peptide, which is specifically binding to the pl-CSA expressed only in the trophoblast cells of placenta, to coat the MTX loaded lipid-polymer nanoparticles (MNPs). Free MTX, MNPs and MNPs coated by a peptide with random sequence of the same amino acids as the pl-CSA binding peptide (SCR-MNPs) were used as control groups. The produced nanoparticles was sphere shape with diameters around 110nm (pl-CSA-MNPs), 100nm (SCR-MNPs) and 70nm (MNPs). Meantime, the biotinylated pl-CSA binding peptide (Bio-pl-CSA) could efficiently bind to mouse and human placental trophoblast cells. Then high-frequency ultrasound (HFUS) was used to monitor the fetuses’ growth in different gorups throughout gestation; meantime Doppler measurements of fetal heart rate (HR) and umbilical blood flow were record to evaluate fetal blood supply and wellbeing. The results showed that, by intravenous injection (i.v) of pl-CSA-MNPs, the conceptus growth was retarded which exhibited a small embryonic cavity. Then, the echodensity of the embryonic fluid was increased and blood flow velocity through the umbilical artery (UA) and vein (UV) was decreased within two days. The corresponding histologic changes are as following fibrinoid changes were surrounded by material granulocytes and lacunae filled with maternal blood were observed at the placenta-maternal interface. In later stages (day 12-14), fetuses showned bradycardia and pericardial edema, ceased development and about 80% fetuses death that was 3 times higher than other groups. The Tunel assay indicated that the placental trophoblast cells apoptosis was increased, while the toxicity of MTX to the liver and kidney was significantly reducing in the pl-CSA-MNPs compare to other groups. Collectively, these results highlight pl-CSA-targeted NPs as a promising and effective alternate regimen for early MA. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10830]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | 2016 |
| 推荐引用方式 GB/T 7714 | Baozhen Zhang,Lunbo Tan,Baobei Wang,et al. Medical Abortion by Trophoblast-Targeted Nanopartciles Loaded with methotrexate[C]. 见:The 4th SKLRB Symposium on Reproductive Biology. 中国北京. |
入库方式： OAI收割
来源：深圳先进技术研究院
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