Collagen-Like Proteins (ClpA, ClpB, ClpC, and ClpD) Are Required for Biofilm Formation and Adhesion to Plant Roots by Bacillus amyloliquefaciens FZB42 (SCI)
文献类型：期刊论文
| 作者 | Zhao X.; Wang Y.; Shang Q. H.; Li Y. Y.; Hao H. T.; Zhang Y. B.; Guo Z. H.; Yang G.; Xie Z. K.; Wang R. Y. |
| 刊名 | Plos One
 |
| 出版日期 | 2015 |
| 卷号 | 10期号:2页码:16 |
| 英文摘要 | The genes of collagen-like proteins (CLPs) have been identified in a broad range of bacteria, including some human pathogens. They are important for biofilm formation and bacterial adhesion to host cells in some human pathogenic bacteria, including several Bacillus spp. strains. Interestingly, some bacterial CLP-encoding genes (clps) have also been found in non-human pathogenic strains such as B. cereus and B. amyloliquefaciens, which are types of plant-growth promoting rhizobacteria (PGPR). In this study, we investigated a putative cluster of clps in B. amyloliquefaciens strain FZB42 and a collagen-related structuralmotif containing glycine-X-threonine repeats was found in the genes RBAM_007740, RBAM_007750, RBAM_007760, and RBAM_007770. Interestingly, biofilm formation was disrupted when these genes were inactivated separately. Scanning electronmicroscopy and hydrophobicity value detection were used to assess the bacterial cell shape morphology and cell surface architecture of clps mutant cells. The results showed that the CLPs appeared to have roles in bacterial autoaggregation, as well as adherence to the surface of abiotic materials and the roots of Arabidopsis thaliana. Thus, we suggest that the CLPs located in the outer layer of the bacterial cell (including the cell wall, outer membrane, flagella, or other associated structures) play important roles in biofilm formation and bacteria-plant interactions. This is the first study to analyze the function of a collagen-like motif-containing protein in a PGPR bacterium. Knocking out each clp gene produced distinctive morphological phenotypes, which demonstrated that each product may play specific roles in biofilm formation. Our in silico analysis suggested that these four tandemly ranked genes might not belong to an operon, but further studies are required at the molecular level to test this hypothesis. These results provide insights into the functions of clps during interactions between bacteria and plants. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.casnw.net/handle/362004/27249]  |
| 专题 | 寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhao X.,Wang Y.,Shang Q. H.,et al. Collagen-Like Proteins (ClpA, ClpB, ClpC, and ClpD) Are Required for Biofilm Formation and Adhesion to Plant Roots by Bacillus amyloliquefaciens FZB42 (SCI)[J]. Plos One,2015,10(2):16. |
| APA | Zhao X..,Wang Y..,Shang Q. H..,Li Y. Y..,Hao H. T..,...&Wang R. Y..(2015).Collagen-Like Proteins (ClpA, ClpB, ClpC, and ClpD) Are Required for Biofilm Formation and Adhesion to Plant Roots by Bacillus amyloliquefaciens FZB42 (SCI).Plos One,10(2),16. |
| MLA | Zhao X.,et al."Collagen-Like Proteins (ClpA, ClpB, ClpC, and ClpD) Are Required for Biofilm Formation and Adhesion to Plant Roots by Bacillus amyloliquefaciens FZB42 (SCI)".Plos One 10.2(2015):16. |
入库方式： OAI收割
来源：寒区旱区环境与工程研究所
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