Molecular characterization of Trimeresurus stejnegeri venom L-amino acid oxidase with potential anti-HIV activity
文献类型：期刊论文
| 作者 | Zhang YJ1,3; Wang JH2,3; Zheng YT[*]2; Zhang Y[*]1; Lee WH1; Wang Q2,3; Liu H1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003 |
| 卷号 | 309期号:3页码:598-604 |
| 关键词 | snake venom L-amino acid oxidase human immunodeficiency virus type 1 antiviral activity Trimeresurus stejnegeri cDNA sequence |
| 通讯作者 | zhengyt@mail.kiz.ac.cn ; zhangy@mail.kiz.ac.cn |
| 英文摘要 | A novel L-amino acid oxidase, named TSV-LAO, has been purified and cloned from the snake Trimeresurus stejnegeri. Fifty percentage cytotoxic concentrations (CC50) of TSV-LAO on C8166 cells were 24 and 390 nM in the absence or presence of catalase (400nM), respectively. However, at concentrations that showed little effect on cell viability, TSV-LAO displayed dose dependent inhibition on HIV-1 infection and replication. The antiviral selectivity indexes (CC50/EC50) were 16 and 6, respectively, corresponding to the measurements of syncytium formation and HIV-1 p24 antigen expression. Interestingly, the presence of catalase resulted in an increase of its antiviral selectivity to 52 and 38. Under the same conditions, no anti-HIV-1 activity was observed by exogenous addition of H2O2. The complete amino acid sequence of TSV-LAO, as deduced from its cDNA, exhibits a high degree of sequence identity with other snake venom LAOs. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of Western Light Project and 10th Five Years Plan pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and Natural Science Foundation of Yunnan Province (99C0085M, 2001C0061M, 2003C0045M) to Dr. Y. Zhang, and CAS Knowledge Innovation Projects (KSCX2-SW-216) to Dr. Y.T. Zheng. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10902]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_分子免疫药理学 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Zhang YJ,Wang JH,Zheng YT[*],et al. Molecular characterization of Trimeresurus stejnegeri venom L-amino acid oxidase with potential anti-HIV activity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,309(3):598-604. |
| APA | Zhang YJ.,Wang JH.,Zheng YT[*].,Zhang Y[*].,Lee WH.,...&Liu H.(2003).Molecular characterization of Trimeresurus stejnegeri venom L-amino acid oxidase with potential anti-HIV activity.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,309(3),598-604. |
| MLA | Zhang YJ,et al."Molecular characterization of Trimeresurus stejnegeri venom L-amino acid oxidase with potential anti-HIV activity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 309.3(2003):598-604. |
入库方式： OAI收割
来源：昆明动物研究所
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