Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters
文献类型：期刊论文
| 作者 | Cui, Yulin ; Qu, Lin; Zhao, Jialin; Qin, Song
|
| 刊名 | PHYCOLOGIA
 |
| 出版日期 | 2016 |
| 卷号 | 55期号:5页码:564-567 |
| 关键词 | Enhanced green fluorescence protein gene Promoter Tetraselmis subcordiformis Transformation rate |
| ISSN号 | 0031-8884 |
| 产权排序 | [Cui, Yulin; Zhao, Jialin; Qin, Song] Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Qu, Lin] State Ocean Adm, Yantai Ocean Environm Monitoring Cent, Yantai 264006, Shandong, Peoples R China; [Zhao, Jialin] Univ Chinese Acad Sci, Beijing 101408, Peoples R China |
| 通讯作者 | Qin, S (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. sqin@yic.ac.cn |
| 英文摘要 | A stable transformation system for Tetraselmis subcordiformis had been established, but a low transformation rate limits its application to this alga. As a promoter is a key factor for transgenic research, a suitable promoter could increase the transformation rate. To improve a transformation system for T. subcordiformis, three commonly used promoters for algal transformation were selected. The CMV, CaMV35S and SV40 promoters were transferred to T. subcordiformis by glass-bead agitation and particle bombardment, with the enhanced green fluorescence protein (egfp) gene as the reporter gene. The transformation rate was calculated according to the GFP fluorescence in transformed algae. Results showed that the CaMV 35S promoter transformation efficiency was higher than that of CMV and SV40 promoters. This report will contribute to efforts to establish a high-efficiency transformation system and expands the fundamental research and applications of this alga. |
| 研究领域[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 关键词[WOS] | ALGA PLATYMONAS-SUBCORDIFORMIS ; CHLAMYDOMONAS-REINHARDTII ; NUCLEAR TRANSFORMATION ; PRASINOPHYCEAE ; AGITATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390261700007 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/19251]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent, Yantai 264006, Shandong, Peoples R China 3.State Ocean Adm, Yantai Ocean Environm Monitoring Cent, Yantai 264006, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 101408, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Yulin,Qu, Lin,Zhao, Jialin,et al. Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters[J]. PHYCOLOGIA,2016,55(5):564-567. |
| APA | Cui, Yulin,Qu, Lin,Zhao, Jialin,&Qin, Song.(2016).Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters.PHYCOLOGIA,55(5),564-567. |
| MLA | Cui, Yulin,et al."Transient expression of the enhanced green fluorescence protein (egfp) gene in Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta) with three exogenous promoters".PHYCOLOGIA 55.5(2016):564-567. |
入库方式： OAI收割
来源：烟台海岸带研究所
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