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Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor
文献类型：期刊论文
| 作者 | Wang, Xiaokun; Choi, Namhyun; Cheng, Ziyi; Ko, Juhui; Chen, Lingxin ; Choo, Jaebum
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2017-01-17 |
| 卷号 | 89期号:2页码:1163-1169 |
| ISSN号 | 0003-2700 |
| 通讯作者 | Choo, J (reprint author), Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea. |
| 产权排序 | [Wang, Xiaokun; Choi, Namhyun; Cheng, Ziyi; Ko, Juhui; Choo, Jaebum] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 英文摘要 | A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposis sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis. |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MULTICOLOR QUANTUM DOTS ; KAPOSIS-SARCOMA ; BACILLARY ANGIOMATOSIS ; QUANTITATIVE DETECTION ; TEST STRIP ; IMMUNOASSAY ; GOLD ; POINT ; AIDS ; NANOPARTICLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392458100021 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/20230]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaokun,Choi, Namhyun,Cheng, Ziyi,et al. Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor[J]. ANALYTICAL CHEMISTRY,2017,89(2):1163-1169. |
| APA | Wang, Xiaokun,Choi, Namhyun,Cheng, Ziyi,Ko, Juhui,Chen, Lingxin,&Choo, Jaebum.(2017).Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor.ANALYTICAL CHEMISTRY,89(2),1163-1169. |
| MLA | Wang, Xiaokun,et al."Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor".ANALYTICAL CHEMISTRY 89.2(2017):1163-1169. |
入库方式： OAI收割
来源：烟台海岸带研究所
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