E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila
文献类型：期刊论文
| 作者 | Zhang, Jing1,2; Tian, Miao1,3,4; Yan, Guan-xiong1,2; Shodhan, Anura3,4; Miao, Wei1 |
| 刊名 | CELL CYCLE
 |
| 出版日期 | 2017 |
| 卷号 | 16期号:1页码:123-135 |
| 关键词 | chromosome segregation DNA repair meiosis Tetrahymena thermophila transcription factor |
| ISSN号 | 1538-4101 |
| 英文摘要 | Members of the E2F family of transcription factors have been reported to regulate the expression of genes involved in cell cycle control, DNA replication, and DNA repair in multicellular eukaryotes. Here, E2FL1, a meiosis-specific E2F transcription factor gene, was identified in the model ciliate Tetrahymena thermophila. Loss of this gene resulted in meiotic arrest prior to anaphase I. The cytological experiments revealed that the meiotic homologous pairing was not affected in the absence of E2FL1, but the paired homologous chromosomes did not separate and assumed a peculiar tandem arrangement. This is the first time that an E2F family member has been shown to regulate meiotic events. Moreover, BrdU incorporation showed that DSB processing during meiosis was abnormal upon the deletion of E2FL1. Transcriptome sequencing analysis revealed that E2FL1 knockout decreased the expression of genes involved in DNA replication and DNA repair in T. thermophila, suggesting that the function of E2F is highly conserved in eukaryotes. In addition, E2FL1 deletion inhibited the expression of related homologous chromosome segregation genes in T. thermophila. The result may explain the meiotic arrest phenotype at anaphase I. Finally, by searching for E2F DNA-binding motifs in the entire T. thermophila genome, we identified 714 genes containing at least one E2F DNA-binding motif; of these, 235 downregulated represent putative E2FL1 target genes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Cell Biology |
| 研究领域[WOS] | Cell Biology |
| 关键词[WOS] | CELL-CYCLE CONTROL ; GENOME-WIDE IDENTIFICATION ; DNA-DAMAGE ; GENE-EXPRESSION ; NUCLEAR-LOCALIZATION ; GAMMA-TUBULIN ; TARGET GENES ; RNA-SEQ ; PROGRESSION ; FAMILY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392737300022 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28880]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria 4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria |
| 推荐引用方式 GB/T 7714 | Zhang, Jing,Tian, Miao,Yan, Guan-xiong,et al. E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila[J]. CELL CYCLE,2017,16(1):123-135. |
| APA | Zhang, Jing,Tian, Miao,Yan, Guan-xiong,Shodhan, Anura,&Miao, Wei.(2017).E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila.CELL CYCLE,16(1),123-135. |
| MLA | Zhang, Jing,et al."E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila".CELL CYCLE 16.1(2017):123-135. |
入库方式： OAI收割
来源：水生生物研究所
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