Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids
文献类型：期刊论文
| 作者 | Zhang, Zhengfeng; Chen, Yanke; Yang, Jun |
| 刊名 | JOURNAL OF MAGNETIC RESONANCE
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 272页码:46-52 |
| 关键词 | Band-selective Heteronuclear recoupling Magic-angle spinning (MAS) solid-state NMR Dual back-to-back pulses (DBP) Double cross polarization (DCP) SPECIFIC CP Membrane proteins Relaxation time in rotating frame (T-1 rho) |
| 英文摘要 | We propose a robust band-selective heteronuclear N-15-C-13 recoupling method using dual back-to-back (BABA) pulses (DBP). It contains four 90 pulses in each rotor period and corresponding phase cycling on each channel (C-13 and N-15). DBP aims at rapid band-selective heteronuclear magnetization transfer between N-15 and C-13 alpha/C-13', whose efficiency is close to that of the well-known SPECIFIC CP in membrane proteins with relatively short relaxation time in rotating frame (T-1,T-rho). Compared to SPECIFIC CP, DBP is very simple to set up and highly robust to RF variations. Thus, it can reduce the efforts in experimental optimization, especially for low-sensitive samples, and is very suitable for long-time or quantitative experiments. The efficacy of DBP is demonstrated by the E. coli diacylglycerol kinase (DAGK) proteoliposome. We anticipate that DBP would be useful for (segments of) membrane proteins that undergo the mu s-ms timescale motions in magic-angle spinning (MAS) solid-state NMR. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biochemical Research Methods ; Physics, Atomic, Molecular & Chemical ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physics ; Spectroscopy |
| 关键词[WOS] | STATE NMR-SPECTROSCOPY ; ANGLE-SPINNING NMR ; DOUBLE-CROSS-POLARIZATION ; ECHO DOUBLE-RESONANCE ; CHEMICAL-SHIFT ASSIGNMENTS ; NUCLEAR-MAGNETIC-RESONANCE ; MAS NMR ; SPECTRAL SIMPLIFICATION ; CONFORMATIONAL-ANALYSIS ; SYMMETRY PRINCIPLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387198100006 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9968]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math,Key Lab Magnet Resonance B, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhengfeng,Chen, Yanke,Yang, Jun. Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids[J]. JOURNAL OF MAGNETIC RESONANCE,2016,272:46-52. |
| APA | Zhang, Zhengfeng,Chen, Yanke,&Yang, Jun.(2016).Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids.JOURNAL OF MAGNETIC RESONANCE,272,46-52. |
| MLA | Zhang, Zhengfeng,et al."Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids".JOURNAL OF MAGNETIC RESONANCE 272(2016):46-52. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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