traditional slanted knife-edge method experiences large errors in the camera modulation transfer function (mtf) due to tilt angle error in the knife-edge resulting in non-uniform sampling of the edge spread function. in order to resolve this problem, a non -uniform sampling knife-edge method for camera mtf measurement is proposed. by applying a simple direct calculation of the fourier transform of the derivative for the non-uniform sampling data, the camera super-sampled mtf results are obtained. theoretical simulations for images with and without noise under different tilt angle errors are run using the proposed method. it is demonstrated that the mtf results are insensitive to tilt angle errors. to verify the accuracy of the proposed method, an experimental setup for camera mtf measurement is established. measurement results show that the proposed method is superior to traditional methods, and improves the universality of the slanted knife-edge method for camera mtf measurement. © 2016 spie.