CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response
文献类型：期刊论文
| 作者 | Liu, Zhaoqun1,3 ; Zhou, Zhi1; Wang, Lingling2; Qiu, Limei1; Zhang, Huan1; Wang, Hao1; Song, Linsheng2
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2016-11-01 |
| 卷号 | 58页码:50-58 |
| 关键词 | Crassostrea gigas Norepinephrine Alpha-1 adrenergic receptor TNF pathway Immune regulation |
| 英文摘要 | We have now cloned an alpha-1 adrenergic receptor (A1AR) from the cDNA library of oyster Crassostrea gigas, designating as CgA1AR-1. The full length of CgA1AR-1 was 1149 bp and it encodes a protein of 382 amino acids containing a 7 transmembrane domain, whose putative topology was similar to the A1ARs in higher organisms and shared similarity of 19% with mammalian A1ARs according to the phylogenic analysis. After cell transfection of CgA1AR-1 into HEK293T cells and the incubation with its specific agonist norepinephrine (NE), the concentration of second messenger Ca2+ increased significantly (p < 0.05). But, this increasing of Ca2+ could be inhibited by adding AJAR antagonist DOX. Tissue distribution assays using qRT-PCR suggested that CgA1AR-1 mRNA was ubiquitously expressed in all the major tissues of oyster. LPS stimulation could induce the up-regulation of CgA1AR-1 mRNA in haemocytes from 12 h to 24 h post stimulation. Moreover, the blocking of CgA1AR-1 by DOX before LPS stimulation affected the mRNA expression of oyster TNF (CGI_10005109 and CGL_10006440) in haemocytes, resulting in the rise of haemocyte phagocytic rate and apoptosis index. In addition to cellular immunity, CgA1AR-1 was also involved in humoral immunity of oyster. Inhibition of CgA1AR-1 with DOX could repress the up-regulation of LZY and SOD activities caused by LPS stimulation. These results suggested that CgA1AR-1 acted as an alpha-1 adrenergic receptor in cetacholaminergic neuroendocrineimmune network mediating both cellular and humoral immune response. (C) 2016 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387523100007 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136200]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:50-58. |
| APA | Liu, Zhaoqun.,Zhou, Zhi.,Wang, Lingling.,Qiu, Limei.,Zhang, Huan.,...&Song, Linsheng.(2016).CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response.FISH & SHELLFISH IMMUNOLOGY,58,50-58. |
| MLA | Liu, Zhaoqun,et al."CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response".FISH & SHELLFISH IMMUNOLOGY 58(2016):50-58. |
入库方式： OAI收割
来源：海洋研究所
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