Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix
文献类型：期刊论文
| 作者 | Wang, Xiudan1,2,3 ; Lin, Hong1; Cao, Limin1; Zheng, Hongwei1; Cui, Mengqi1; Du, Shuyuan1; Sui, Jianxin1
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| 刊名 | FOOD SCIENCE AND BIOTECHNOLOGY
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| 出版日期 | 2016-10-01 |
| 卷号 | 25期号:5页码:1265-1273 |
| 关键词 | matrix interference fish protein enzyme-linked immunosorbent assay biochemical property aldolase |
| 英文摘要 | The interaction between some proteins and immune globulin has been confirmed as an important source of matrix interference with the immunoassay of fishery products, but detailed biochemical properties of these proteins have not been indicated. Two interference-inducing proteins (42 and 36 kD) in flounder were isolated, characterized, and identified. Their influences on the immunoassay of norfloxacin were confirmed by western blotting and enzyme-linked immunosorbent assay. The pI value and pH stability of the two proteins were also investigated. Using LC-MS/MS, the two proteins were identified as fructose-bisphosphate aldolase, and such results were partly verified by the aldolase activity of the 42 and 36 kD isolates. Considering the prevalence of these proteins (as multi-functional aldolase of muscles) in foods, these results would help to further understand the matrix effects in various immunoassays as well as the development of effective techniques to improve the efficiency of immunoassays. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387529400006 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136210]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Food Safety Lab, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiudan,Lin, Hong,Cao, Limin,et al. Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2016,25(5):1265-1273. |
| APA | Wang, Xiudan.,Lin, Hong.,Cao, Limin.,Zheng, Hongwei.,Cui, Mengqi.,...&Sui, Jianxin.(2016).Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix.FOOD SCIENCE AND BIOTECHNOLOGY,25(5),1265-1273. |
| MLA | Wang, Xiudan,et al."Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix".FOOD SCIENCE AND BIOTECHNOLOGY 25.5(2016):1265-1273. |
入库方式： OAI收割
来源：海洋研究所
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