Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase
文献类型：期刊论文
| 作者 | Wang, Xueyan1; Duan, Delin2; Fu, Xiaoting1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 93页码:600-608 |
| 关键词 | Arylsulfatase agarose desulfation sulfated galactan spectroscopy |
| 英文摘要 | Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-L-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-L-galactose, 2-O-methyl-3,6-anhydro-L-galactose and xylose. Data from C-13 NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of I-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively. (C) 2016 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389090900070 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136232]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xueyan,Duan, Delin,Fu, Xiaoting. Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,93:600-608. |
| APA | Wang, Xueyan,Duan, Delin,&Fu, Xiaoting.(2016).Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,93,600-608. |
| MLA | Wang, Xueyan,et al."Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 93(2016):600-608. |
入库方式： OAI收割
来源：海洋研究所
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