The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas
文献类型：期刊论文
| 作者 | Wang, Weilin1,3 ; Li, Meijia1,3; Wang, Lingling2; Chen, Hao1,3; Liu, Zhaoqun1,3; Jia, Zhihao1,3; Qiu, Limei1; Song, Linsheng1,2
|
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 67页码:221-228 |
| 关键词 | Crassostrea gigas Hemocyte separation Granulocytes Immunocompetent Functional features |
| 英文摘要 | Hemocytes comprise diverse cell types with morphological and functional heterogeneity and play indispensable roles in immunological homeostasis of invertebrates. The morphological classification of different hemocytes in mollusk has been studied since the 1970's, yet the involvement of the different sub-populations in immune functions is far from clear. In the present study, three types of hemocytes were morphologically identified and separated as agranulocytes, semi-granulocytes and granulocytes by flow cytometry and Percoll density gradient centrifugation. The granulocytes were characterized functionally as the main phagocytic and encapsulating population, while semi-granulocytes and agranulocytes exhibited low or no such capacities, respectively. Meanwhile, the lysosome activity and the productions of ROS and NO were all mainly concentrated in granulocytes under both normal and immune-activated situations. Further, the mRNA transcripts of some immune related genes, including CgTLR, CgClathrin, CgATPeV, CgLysozyme, CgDefensin and CgIL-17, were mainly expressed in granulocytes, lower in semi-granulocytes and agranulocytes. These results collectively suggested that the granulocytes were the main immunocompetent hemocytes in oyster C. gigas, and a differentiation relationship among these three sub-population hemocytes was inferred based on the gradual changes in morphological, functional and molecular features. (C) 2016 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390071200021 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136251]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Weilin,Li, Meijia,Wang, Lingling,et al. The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:221-228. |
| APA | Wang, Weilin.,Li, Meijia.,Wang, Lingling.,Chen, Hao.,Liu, Zhaoqun.,...&Song, Linsheng.(2017).The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,221-228. |
| MLA | Wang, Weilin,et al."The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):221-228. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。