Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas
文献类型：期刊论文
| 作者 | Jiang, Shuai1; Jia, Zhihao1; Zhang, Tao1 ; Wang, Lingling1,2; Qiu, Limei1; Sun, Jinsheng3; Song, Linsheng2
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| 刊名 | PEERJ
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| 出版日期 | 2016-12-14 |
| 卷号 | 4 |
| 关键词 | Crassostrea gigas Phagocytosis Flow cytometry Lectin staining Polylactosamine |
| 英文摘要 | Invertebrates lack canonical adaptive immunity and mainly rely on innate immune system to fight against Pathogens. The phagocytes, which could engulf and kill microbial pathogens, are likely to be of great importance and have to undertake significant roles in invertebrate immune defense. In the present study, flow cytometry combined with histological and lectin staining was employed to characterise functional features of phagocytes in the Pacific oyster Crassostrea gigas. Based on the cell size and celkilar contents, haemocytes were categorised into three cell types, i.e., granulocytes, semigranulocytes and agranulocytes. Agranulocytes with smaller cell volume and lower cytoplasmic-to-nuclear ratio did not show phagocytic activity, while semigranulocytes and agranulocytes exhibited larger cell volume, higher cytoplasmic-to-nuclear ratio and phagocytic activity. In addition, granulocytes with higher side scatter (SSC) exhibited higher phagocytic activity than that of semigranulocytes. When beta-integrin and lectinlike receptors were blocked by RGD tripeptide and carbohydrates, respectively, the phagocytic activity of both granulocytes and semigranulocytes was significantly inhibited, indicating that,beta-integrin and certain lectin-like receptors were involved in phagocytosis towards microbes. Moreover, lipopolysaccharide but not peptidylglycan could enhance phagocytic activity of granulocytes and semigranulocytes towards Vibrio splendidus and Staphyloeoccus aureus. Lectin staining analysis revealed that Lyeopersicou esculentum lectin (LEL), binding the epitope polylactosaniine, was highly distributed on the extracellular cell surface of phagocytes, and could be utilized as a potential molecular marker to differentiate phagocytes from non-phagocytic haemocytes. The results, collectively, provide knowledge on the functional characters of oyster phagocytes, which would contribute to deep investigation of cell typing and cellular immunity in bivalves. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390054600001 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136256]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian, Liaoning, Peoples R China 3.Tianjin Normal Univ, Tianjin Key Lab Anim & Plant Resistance, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Shuai,Jia, Zhihao,Zhang, Tao,et al. Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas[J]. PEERJ,2016,4. |
| APA | Jiang, Shuai.,Jia, Zhihao.,Zhang, Tao.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2016).Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas.PEERJ,4. |
| MLA | Jiang, Shuai,et al."Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas".PEERJ 4(2016). |
入库方式： OAI收割
来源：海洋研究所
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