Demineralized Bone Matrix Scaffolds Modified by CBD-SDF-1 alpha Promote Bone Regeneration via Recruiting Endogenous Stem Cells
文献类型：期刊论文
| 作者 | Shi, JJ(施家佳); Sun, J(孙杰); Zhang, W; Liang, H(梁辉); Shi, Q; Li, XR(李晓然); Chen, YY(陈艳艳); Zhuang, Y(庄燕); Dai, JW(戴建武) |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016 |
| 卷号 | 8期号:41 |
| 通讯作者 | Dai, JW(戴建武) |
| 英文摘要 | The reconstruction of bone usually depends on substitute transplantation, which has drawbacks including the limited bone substitutes available, comorbidity, immune rejection, and limited endogenous bone regeneration. Here, we constructed a functionalized bone substitute by combining application of the demineralized bone matrix (DBM) and collagen-binding stromal-cell-derived factor-1 alpha (CBD-SDF-1 alpha). DBM was a poriferous and biodegradable bone substitute, derived from bovine bone and consisting mainly of collagen. CBD-SDF-1 alpha could bind to collagen and be controllably released from the DBM to mobilize stem cells. In a rat femur defect model, CBD-SDF-1 alpha-modified DBM scaffolds could efficiently mobilize CD34(+) and c-kit(+) endogenous stem cells homing to the injured site at 3 days after implantation. According to the data from micro-CT, CBD-SDF-1 alpha-modified DBM scaffolds could help the bone defects rejoin with mineralization accumulated and bone volume expanded. Interestingly, osteoprotegerin (OPG) and osteopontin (OPN) were highly expressed in CBD-SDF-1 alpha group at an early time after implantation, while osteocalcin (OCN) was more expanded. H&E and Massons trichrome staining showed that the CBD-SDF-1 alpha-modified DBM scaffold group had more osteoblasts and that the bone defect rejoined earlier. The ultimate strength of the regenerated bone was investigated by three-point bending, showing that the CBD-SDF-1 alpha group had superior strength. In conclusion, CBD-SDF-1 alpha -modified DBM scaffolds could promote bone regeneration by recruiting endogenous stem cells. |
| 关键词[WOS] | BETA-TRICALCIUM PHOSPHATE ; OSTEOGENIC DIFFERENTIATION ; MORPHOGENETIC PROTEIN-2 ; CALCIUM-PHOSPHATE ; BINDING ; SDF-1-ALPHA ; REPAIR ; ARTHRITIS ; NETWORKS ; STRENGTH |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000385992400014 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4722]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 推荐引用方式 GB/T 7714 | Shi, JJ,Sun, J,Zhang, W,et al. Demineralized Bone Matrix Scaffolds Modified by CBD-SDF-1 alpha Promote Bone Regeneration via Recruiting Endogenous Stem Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(41). |
| APA | Shi, JJ.,Sun, J.,Zhang, W.,Liang, H.,Shi, Q.,...&Dai, JW.(2016).Demineralized Bone Matrix Scaffolds Modified by CBD-SDF-1 alpha Promote Bone Regeneration via Recruiting Endogenous Stem Cells.ACS APPLIED MATERIALS & INTERFACES,8(41). |
| MLA | Shi, JJ,et al."Demineralized Bone Matrix Scaffolds Modified by CBD-SDF-1 alpha Promote Bone Regeneration via Recruiting Endogenous Stem Cells".ACS APPLIED MATERIALS & INTERFACES 8.41(2016). |
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