A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix
文献类型：期刊论文
| 作者 | Liang, H(梁辉); Li, XR(李晓然); Wang, B; Chen, B; Zhao, YN; Sun, J(孙杰); Zhuang, Y(庄燕); Shi, JJ(施家佳); Shen, H(沈贺); Zhang, ZJ(张智军) |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 卷号 | 6 |
| 通讯作者 | Dai, JW(戴建武) |
| 英文摘要 | Many tumors over-express collagen, which constitutes the physical scaffold of tumor microenvironment. Collagen has been considered to be a target for cancer therapy. The collagen-binding domain (CBD) is a short peptide, which could bind to collagen and achieve the sustained release of CBD-fused proteins in collagen scaffold. Here, a collagen-binding EGFR antibody fragment was designed and expressed for targeting the collagen-rich extracellular matrix in tumors. The antibody fragment (Fab) of cetuximab was fused with CBD (CBD-Fab) and expressed in Pichia pastoris. CBD-Fab maintained antigen binding and anti-tumor activity of cetuximab and obtained a collagen-binding ability in vitro. The results also showed CBD-Fab was mainly enriched in tumors and had longer retention time in tumors in A431 s.c. xenografts. Furthermore, CBD-Fab showed a similar therapeutic efficacy as cetuximab in A431 xenografts. Although CBD-Fab hasn't showed better therapeutic effects than cetuximab, its smaller molecular and special target may be applicable as antibody-drug conjugates (ADC) or immunotoxins. |
| 关键词[WOS] | GROWTH-FACTOR RECEPTOR ; PICHIA-PASTORIS ; MONOCLONAL-ANTIBODIES ; CANCER-THERAPY ; IN-VIVO ; MICROENVIRONMENTAL REGULATION ; PROGRESSION ; EFFICACY ; INVASION ; MECHANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370234500001 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4784]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 推荐引用方式 GB/T 7714 | Liang, H,Li, XR,Wang, B,et al. A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Liang, H.,Li, XR.,Wang, B.,Chen, B.,Zhao, YN.,...&Dai, JW.(2016).A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix.SCIENTIFIC REPORTS,6. |
| MLA | Liang, H,et al."A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
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