Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry
文献类型：期刊论文
| 作者 | Huang, CH; Yang, QL; Song, FY; Chen, N; Yao, B; Liao, XL; Zhang, ST; Chen, YY(陈艳艳); Jin, G |
| 刊名 | INTEGRATED FERROELECTRICS
 |
| 出版日期 | 2016 |
| 卷号 | 171期号:1 |
| 通讯作者 | Huang, CH ; Chen, YY(陈艳艳) ; Jin, G |
| 英文摘要 | This study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0-100.0 U/mL, 0.1-64.0 ng/mL, 1.0-128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1-7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60min for an automated process. The proposed assay has the potential for practical application. |
| 关键词[WOS] | HEPATOCELLULAR-CARCINOMA ; VISUALIZATION ; DIAGNOSIS ; ANTIGENS ; MARKERS ; CANCER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378406600009 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4849]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 推荐引用方式 GB/T 7714 | Huang, CH,Yang, QL,Song, FY,et al. Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2016,171(1). |
| APA | Huang, CH.,Yang, QL.,Song, FY.,Chen, N.,Yao, B.,...&Jin, G.(2016).Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,171(1). |
| MLA | Huang, CH,et al."Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 171.1(2016). |
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