Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor
文献类型：期刊论文
| 作者 | Huang, CH; Yang, QL; Song, FY; Chen, N; Liao, XL; Yao, B; Zhang, ST; Chen, YY(陈艳艳); Jin, G |
| 刊名 | INTEGRATED FERROELECTRICS
 |
| 出版日期 | 2016 |
| 卷号 | 171期号:1 |
| 通讯作者 | Huang, CH ; Chen, YY(陈艳艳) ; Jin, G |
| 英文摘要 | Carbohydrate side chain in antibody Fc region can be oxidized into aldehyde group by sodium m-periodate, which can simultaneously couple with the amino-group on silicon surface obtained by chemistry modification. This combination will introduce C=N linkage and site-directed immobilization of antibody, which was confirmed by AFM observation. The acquired antibody layer can then be used for immunoassay for alpha-fetoprotein (AFP). The combined antigen is directly detected by imaging ellipsometry in grayscale format. The proposed method was further compared with commercial kit by determination 20 clinic samples with significant correlation (R = 0.93). |
| 关键词[WOS] | ATOMIC-FORCE MICROSCOPY ; IMAGING ELLIPSOMETRY ; VISUALIZATION |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378406600010 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4850]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 推荐引用方式 GB/T 7714 | Huang, CH,Yang, QL,Song, FY,et al. Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor[J]. INTEGRATED FERROELECTRICS,2016,171(1). |
| APA | Huang, CH.,Yang, QL.,Song, FY.,Chen, N.,Liao, XL.,...&Jin, G.(2016).Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor.INTEGRATED FERROELECTRICS,171(1). |
| MLA | Huang, CH,et al."Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor".INTEGRATED FERROELECTRICS 171.1(2016). |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。