Selection and analysis of DNA aptamers to berberine to develop a label-free light-up fluorescent probe
文献类型：期刊论文
| 作者 | Wang, JE(王金娥); Zhang, YJ; Wang, HY(王红沿); Chen, Y(陈阳); Xu, LJ(许丽君); Gao, T; Pei, RJ(裴仁军) |
| 刊名 | NEW JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 40期号:11 |
| 通讯作者 | Pei, RJ(裴仁军) |
| 英文摘要 | Aptamers are short single-stranded DNA or RNA, which can be selected from a random combinatorial library by the systematic evolution of ligands by exponential enrichment (SELEX) in vitro. In order to obtain novel selective light-up ligands for berberine, we selected DNA aptamers for berberine by a modified affinity chromatography-based SELEX. The ssDNA library was coupled on streptavidin-coated agarose beads through the hybridization with a biotin labeled complementary oligonucleotide capture strand, and the aptamer sequences were released from agarose beads through berberine binding. This method to isolate the aptamers was simple, straightforward and effective, and three aptamers with low micromolar dissociation constants (K(d)s) were achieved after only 6 rounds of selection. The best of them was further optimized, and the shortened aptamers showed similar K(d)s to the full length sequences. The light-up phenomenon of the berberine-aptamer pair was also investigated and the final minimized 21-mer aptamer which showed higher fluorescence enhancement than G-quadruplex structures could be used as a novel light-up fluorescent probe for various biosensing applications. |
| 关键词[WOS] | G-QUADRUPLEX DNA ; IN-VITRO ; MALACHITE GREEN ; DOWN-REGULATION ; RNA ; DERIVATIVES ; LIGANDS ; BINDING ; DYES ; INHIBITION |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387621500091 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4681]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_裴仁军团队 |
| 推荐引用方式 GB/T 7714 | Wang, JE,Zhang, YJ,Wang, HY,et al. Selection and analysis of DNA aptamers to berberine to develop a label-free light-up fluorescent probe[J]. NEW JOURNAL OF CHEMISTRY,2016,40(11). |
| APA | Wang, JE.,Zhang, YJ.,Wang, HY.,Chen, Y.,Xu, LJ.,...&Pei, RJ.(2016).Selection and analysis of DNA aptamers to berberine to develop a label-free light-up fluorescent probe.NEW JOURNAL OF CHEMISTRY,40(11). |
| MLA | Wang, JE,et al."Selection and analysis of DNA aptamers to berberine to develop a label-free light-up fluorescent probe".NEW JOURNAL OF CHEMISTRY 40.11(2016). |
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