Selection and characterization of DNA aptamers for the development of light-up biosensor to detect Cd(II)
文献类型：期刊论文
| 作者 | Wang, HY(王红沿); Cheng, H(程慧); Wang, J(王金娥); Xu, LJ; Chen, HX; Pei, RJ(裴仁军) |
| 刊名 | TALANTA
 |
| 出版日期 | 2016 |
| 卷号 | 154 |
| 通讯作者 | Pei, RJ(裴仁军) |
| 英文摘要 | In order to develop a facile, cost-effective and quick-testing light-up biosensor with excellent specificity for cadmium ions (Cd(II)) detection, a modified selection method based on target-induced release of strands was used to isolate aptamers of Cd (II) with high specificity. Circular Dichroism (CD) data confirmed that one of the selected aptamers underwent a distinct conformational change on addition of Cd (II). A biosensor for Cd(II) was developed based on the Cd(II)-induced release of fluorescence-labeled aptamer from complex with a quencher-labeled short complementary sequence. The sensing platform displayed a Cd(II) concentration -dependent increase of fluorescence intensity in the low micromolar range and had an excellent selectivity in the presence of various interfering metal ions. Such biosensor could potentially be used for the detection of Cd(II) in environmental samples. (C) 2016 Elsevier B.V. All rights reserved. |
| 关键词[WOS] | IN-VITRO SELECTION ; SOLID-PHASE EXTRACTION ; NUCLEIC-ACID APTAMERS ; AQUEOUS-SOLUTION ; METAL-IONS ; SIGNALING APTAMERS ; ICP-MS ; CADMIUM ; LEAD ; RNA |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376696400066 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4685]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_裴仁军团队 |
| 推荐引用方式 GB/T 7714 | Wang, HY,Cheng, H,Wang, J,et al. Selection and characterization of DNA aptamers for the development of light-up biosensor to detect Cd(II)[J]. TALANTA,2016,154. |
| APA | Wang, HY,Cheng, H,Wang, J,Xu, LJ,Chen, HX,&Pei, RJ.(2016).Selection and characterization of DNA aptamers for the development of light-up biosensor to detect Cd(II).TALANTA,154. |
| MLA | Wang, HY,et al."Selection and characterization of DNA aptamers for the development of light-up biosensor to detect Cd(II)".TALANTA 154(2016). |
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