Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene
文献类型：期刊论文
| 作者 | Hong, Shanni(洪珊妮); Sun, Na(孙娜); Liu, Min(刘敏); Wang, Jine(王金娥); Pei, Renjun(裴仁军) |
| 刊名 | RSC Advances
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:113 |
| 通讯作者 | Liu, M(刘敏) ; Pei, RJ(裴仁军) |
| 英文摘要 | Galectin-1 is closely related with immune systems, and its overexpression may cause tumor metastasis. Owing to the better stability of antisense oligonucleotides than siRNA, we constructed a chimera of aptamer-antisense oligonucleotide (Apt-AS) for silencing the galectin-1 gene specifically. For studying the endocytosis pathways of Apt-AS by aptamer targeted delivery, we marked the 3' terminus of Apt-AS with Alexa Flour 488 (Apt-AS488) so as to trace the pathways through the confocal microscope. Furthermore, Apt-AS was used to silence the expression of galectin-1. The results showed Apt-AS entered cells by a caveolae mediated endocytosis pathway, and the aptamer-antisense chimeras did not impair the antisense gene silencing efficiency. Moreover Apt-AS could improve the cellular uptake and selectively entered cells. |
| 关键词[WOS] | TARGETED CANCER-THERAPY ; INTRACELLULAR DELIVERY ; DNA NANOSTRUCTURES ; IN-VIVO ; SIRNA ; CELLS ; EFFICIENT ; VEHICLES |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389708700092 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4861]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_裴仁军团队 |
| 推荐引用方式 GB/T 7714 | Hong, Shanni,Sun, Na,Liu, Min,et al. Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene[J]. RSC Advances,2016,6(113). |
| APA | Hong, Shanni,Sun, Na,Liu, Min,Wang, Jine,&Pei, Renjun.(2016).Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene.RSC Advances,6(113). |
| MLA | Hong, Shanni,et al."Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene".RSC Advances 6.113(2016). |
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